Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1- 3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B
it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with DnaC protein, primase, and other prepriming proteins
K02314
-
3.6.4.12
1.907e-212
670.0
MMGS2_k127_1173035_2
Bacterial extracellular solute-binding proteins, family 5 Middle
Subunits I and II form the functional core of the enzyme complex. Electrons originating in cytochrome c are transferred via heme a and Cu(A) to the binuclear center formed by heme a3 and Cu(B)
Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group
A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner
K02469
-
5.99.1.3
0.0
1364.0
MMGS2_k127_1196478_1
AcrB/AcrD/AcrF family
K18138
-
-
0.0
1307.0
MMGS2_k127_1196478_10
Type VI secretion protein, EvpB/VC_A0108, tail sheath
The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision
K03703
-
-
5.358e-260
814.0
MMGS2_k127_1196478_110
Binds to Cpn60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter
One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it helps nucleate assembly of the platform of the 30S subunit by binding and bridging several RNA helices of the 16S rRNA
K02956
-
-
0.00000000000000000000000000000000000149
139.0
MMGS2_k127_1196478_115
PFAM Excinuclease ABC, C subunit
K07461
-
-
0.00000000000000000000000000000000002101
136.0
MMGS2_k127_1196478_116
Binds as a heterodimer with protein S6 to the central domain of the 16S rRNA, where it helps stabilize the platform of the 30S subunit
K02963
-
-
0.00000000000000000000000000000000002832
136.0
MMGS2_k127_1196478_117
Involved in formation and maintenance of cell shape
K03570
-
-
0.0000000000000000000000000000000001962
143.0
MMGS2_k127_1196478_118
Acts on guanine, xanthine and to a lesser extent hypoxanthine
Part of the MsrPQ system that repairs oxidized periplasmic proteins containing methionine sulfoxide residues (Met-O), using respiratory chain electrons. Thus protects these proteins from oxidative-stress damage caused by reactive species of oxygen and chlorine generated by the host defense mechanisms. MsrPQ is essential for the maintenance of envelope integrity under bleach stress, rescuing a wide series of structurally unrelated periplasmic proteins from methionine oxidation. MsrQ provides electrons for reduction to the reductase catalytic subunit MsrP, using the quinone pool of the respiratory chain
K17247
-
-
0.0000000000000000000000000000000006853
137.0
MMGS2_k127_1196478_12
Electron transfer flavoprotein-ubiquinone oxidoreductase, 4Fe-4S
K00311
-
1.5.5.1
1.583e-254
793.0
MMGS2_k127_1196478_120
Acetyltransferase (GNAT) domain
-
-
-
0.000000000000000000000000000000002639
133.0
MMGS2_k127_1196478_121
Tetratricopeptide repeat
-
-
-
0.000000000000000000000000000000003483
146.0
MMGS2_k127_1196478_123
Putative bacterial sensory transduction regulator
-
-
-
0.0000000000000000000000000000003686
128.0
MMGS2_k127_1196478_125
Patatin-like phospholipase
-
-
-
0.000000000000000000000000000000693
138.0
MMGS2_k127_1196478_126
Flavin reductase like domain
-
-
-
0.000000000000000000000000000004017
124.0
MMGS2_k127_1196478_127
Cold shock
K03704
-
-
0.0000000000000000000000000007632
116.0
MMGS2_k127_1196478_129
-O-antigen
-
-
-
0.0000000000000000000000007485
121.0
MMGS2_k127_1196478_13
Transglycosylase
K05366
-
2.4.1.129,3.4.16.4
5.021e-250
789.0
MMGS2_k127_1196478_130
Scp-like extracellular
-
-
-
0.00000000000000000000005019
104.0
MMGS2_k127_1196478_131
Co-chaperone involved in the maturation of iron-sulfur cluster-containing proteins. Seems to help targeting proteins to be folded toward HscA
Interacts with outer membrane receptor proteins that carry out high-affinity binding and energy dependent uptake into the periplasmic space of specific substrates. It could act to transduce energy from the cytoplasmic membrane to specific energy- requiring processes in the outer membrane, resulting in the release into the periplasm of ligands bound by these outer membrane proteins
K03832
-
-
0.00000001492
64.0
MMGS2_k127_1196478_14
Involved in the restart of stalled replication forks. Recognizes and binds the arrested nascent DNA chain at stalled replication forks. It can open the DNA duplex, via its helicase activity, and promote assembly of the primosome and loading of the major replicative helicase DnaB onto DNA
K04066
-
-
4.232e-245
773.0
MMGS2_k127_1196478_140
Outer membrane protein beta-barrel domain
-
-
-
0.0000003506
60.0
MMGS2_k127_1196478_141
PFAM peptidase S1 and S6, chymotrypsin Hap
-
-
-
0.0000003671
62.0
MMGS2_k127_1196478_142
-
-
-
-
0.00000142
52.0
MMGS2_k127_1196478_143
Protein of unknown function (DUF465)
-
-
-
0.000002657
52.0
MMGS2_k127_1196478_146
Domain of unknown function (DUF4167)
-
-
-
0.0002281
47.0
MMGS2_k127_1196478_149
-
-
-
-
0.0005593
49.0
MMGS2_k127_1196478_15
it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box) 5'-TTATC CA A CA A-3'. DnaA binds to ATP and to acidic phospholipids
K02313
-
-
1.704e-219
689.0
MMGS2_k127_1196478_16
Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)
K01885
-
6.1.1.17
2.242e-219
688.0
MMGS2_k127_1196478_17
Dehydrogenase
K00382
-
1.8.1.4
1.541e-213
671.0
MMGS2_k127_1196478_18
Belongs to the citrate synthase family
K01647
-
2.3.3.1
9.327e-210
661.0
MMGS2_k127_1196478_19
Master enzyme that delivers sulfur to a number of partners involved in Fe-S cluster assembly, tRNA modification or cofactor biosynthesis. Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Functions as a sulfur delivery protein for Fe-S cluster synthesis onto IscU, an Fe-S scaffold assembly protein, as well as other S acceptor proteins
K04487
-
2.8.1.7
1.134e-208
653.0
MMGS2_k127_1196478_2
In addition to polymerase activity, this DNA polymerase exhibits 5'-3' exonuclease activity
K02335
-
2.7.7.7
0.0
1133.0
MMGS2_k127_1196478_20
Chaperone involved in the maturation of iron-sulfur cluster-containing proteins. Has a low intrinsic ATPase activity which is markedly stimulated by HscB
Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF- independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism
Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage
Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives
Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria
A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner
Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction
K00962
-
2.7.7.8
0.0
1006.0
MMGS2_k127_1196478_40
Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib-5-P)
Proton pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for proton movement across the membrane. Generates a proton motive force
Catalyzes the prenylation of para-hydroxybenzoate (PHB) with an all-trans polyprenyl group. Mediates the second step in the final reaction sequence of ubiquinone-8 (UQ-8) biosynthesis, which is the condensation of the polyisoprenoid side chain with PHB, generating the first membrane-bound Q intermediate 3- octaprenyl-4-hydroxybenzoate
IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins
Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group
A scaffold on which IscS assembles Fe-S clusters. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters
Plays an important role in DNA replication, recombination and repair. Binds to ssDNA and to an array of partner proteins to recruit them to their sites of action during DNA metabolism
Attaches a formyl group to the free amino group of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus
Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides
Attaches a formyl group to the free amino group of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus
Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions
Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE
K03695
-
-
0.0
1270.0
MMGS2_k127_1363821_1
ImcF-related N-terminal domain
K11891
-
-
1.265e-309
987.0
MMGS2_k127_1363821_10
ATP-dependent carboxylate-amine ligase which exhibits weak glutamate--cysteine ligase activity
Catalyzes the hydrolysis of UDP-3-O-myristoyl-N- acetylglucosamine to form UDP-3-O-myristoylglucosamine and acetate, the committed step in lipid A biosynthesis
Catalyzes the specific phosphorylation of 1,6-anhydro-N- acetylmuramic acid (anhMurNAc) with the simultaneous cleavage of the 1,6-anhydro ring, generating MurNAc-6-P. Is required for the utilization of anhMurNAc either imported from the medium or derived from its own cell wall murein, and thus plays a role in cell wall recycling
Bacterial extracellular solute-binding proteins, family 5 Middle
K13893
-
-
4.044e-221
703.0
MMGS2_k127_1363821_20
Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another
Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)
Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome
Catalyzes the reversible interconversion of isobutyryl- CoA and n-butyryl-CoA, using radical chemistry. Also exhibits GTPase activity, associated with its G-protein domain (MeaI) that functions as a chaperone that assists cofactor delivery and proper holo-enzyme assembly
K01847
-
5.4.99.2
4.914e-296
923.0
MMGS2_k127_1449956_1
Glycyl-tRNA synthetase beta subunit
K01879
-
6.1.1.14
5.358e-205
661.0
MMGS2_k127_1449956_10
Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and or degradation of damaged proteins
once thought to export heme, this seems not to be the case, but its exact role is uncertain. Responsible for energy coupling to the transport system
K02193
-
3.6.3.41
0.000000000000000000000000000000000009329
143.0
MMGS2_k127_1449956_13
Heme chaperone required for the biogenesis of c-type cytochromes. Transiently binds heme delivered by CcmC and transfers the heme to apo-cytochromes in a process facilitated by CcmF and CcmH
K02197
-
-
0.00000000000000000000000000000000003316
140.0
MMGS2_k127_1449956_14
ATPase with chaperone activity
K07391
-
-
0.0000000000000000000000000000000000465
137.0
MMGS2_k127_1449956_15
haloacid dehalogenase-like hydrolase
K01091
-
3.1.3.18
0.00000000000000000000000001041
117.0
MMGS2_k127_1449956_16
Mechanosensitive ion channel
-
-
-
0.000000000000000000000002512
118.0
MMGS2_k127_1449956_17
Required for the export of heme to the periplasm for the biogenesis of c-type cytochromes
helix_turn_helix multiple antibiotic resistance protein
-
-
-
0.00000000000000000000003807
105.0
MMGS2_k127_1502933_2
Protein of unknown function (DUF1398)
-
-
-
0.0000000878
56.0
MMGS2_k127_1539216_0
Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner
K03596
-
-
0.0
1058.0
MMGS2_k127_1539216_1
Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily
K01890
-
6.1.1.20
9.437e-284
892.0
MMGS2_k127_1539216_2
Transfers the N-acyl diglyceride group on what will become the N-terminal cysteine of membrane lipoproteins
Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule
K02621
-
-
0.0
1069.0
MMGS2_k127_1547441_1
Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP)
K01662
-
2.2.1.7
1.489e-305
947.0
MMGS2_k127_1547441_10
One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit
Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate
Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides
Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and or for immediate growth after restoration of oxygen
Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain
Required for rescue of stalled ribosomes mediated by trans-translation. Binds to transfer-messenger RNA (tmRNA), required for stable association of tmRNA with ribosomes. tmRNA and SmpB together mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is encoded by the ssrA gene
Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family
K18138,K18989
-
-
0.0
1215.0
MMGS2_k127_1706250_1
DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA
Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1- P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA)
Required for disulfide bond formation in some periplasmic proteins. Acts by transferring its disulfide bond to other proteins and is reduced in the process
Part of an ABC transporter complex. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP- binding domain (NBD) is responsible for energy generation (By similarity)
Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates
K01937
-
6.3.4.2
3.098e-267
830.0
MMGS2_k127_192161_1
HI0933-like protein
K07007
-
-
2.148e-194
612.0
MMGS2_k127_192161_10
Involved in protein export. Participates in an early event of protein translocation (By similarity)
K03075
-
-
0.0000000000000000000000000138
112.0
MMGS2_k127_192161_11
Septum formation initiator
-
-
-
0.000000000000000000000005449
105.0
MMGS2_k127_192161_12
Belongs to the 'phage' integrase family
-
-
-
0.0000000000000000000004512
98.0
MMGS2_k127_192161_13
Acetyltransferase (GNAT) domain
-
-
-
0.00000000000000000001357
96.0
MMGS2_k127_192161_14
Mechanosensitive ion channel
K22044
-
-
0.00000000000000927
76.0
MMGS2_k127_192161_15
COGs COG3677 Transposase and inactivated derivatives
K07480
-
-
0.00002557
46.0
MMGS2_k127_192161_16
first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA
-
-
-
0.0001084
54.0
MMGS2_k127_192161_2
Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction the enzyme is irreversibly inactivated
Acts both as a biotin-- acetyl-CoA-carboxylase ligase and a biotin-operon repressor. In the presence of ATP, BirA activates biotin to form the BirA-biotinyl-5'-adenylate (BirA-bio- 5'-AMP or holoBirA) complex. HoloBirA can either transfer the biotinyl moiety to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase, or bind to the biotin operator site and inhibit transcription of the operon
Oxidizes proline to glutamate for use as a carbon and nitrogen source
K13821
-
1.2.1.88,1.5.5.2
0.0
1157.0
MMGS2_k127_196482_1
Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins
Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits
Catalyzes the reversible conversion of 3- phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4- phosphonooxybutanoate to phosphohydroxythreonine
Involved in the heme biosynthesis. Catalyzes the aerobic oxidative decarboxylation of propionate groups of rings A and B of coproporphyrinogen-III to yield the vinyl groups in protoporphyrinogen-IX
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates
K03043
-
2.7.7.6
0.0
2294.0
MMGS2_k127_2476007_1
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates
K03046,K13797
-
2.7.7.6
0.0
2279.0
MMGS2_k127_2476007_10
SecE/Sec61-gamma subunits of protein translocation complex
K03073
-
-
0.00000000000000000005256
91.0
MMGS2_k127_2476007_11
This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis
K02358
-
-
0.00000000000000000006197
89.0
MMGS2_k127_2476007_12
Elongation factor Tu C-terminal domain
K02358
-
-
0.00000000000000467
74.0
MMGS2_k127_2476007_2
Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome
K02355
-
-
0.0
1151.0
MMGS2_k127_2476007_3
Binds directly to 23S rRNA. The L1 stalk is quite mobile in the ribosome, and is involved in E site tRNA release
Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit
One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, probably blocks exit of the E-site tRNA
Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA
K01895
-
6.2.1.1
6.574e-288
895.0
MMGS2_k127_2621582_10
Catalyzes the transfer of an acyl group from acyl- phosphate (acyl-PO(4)) to glycerol-3-phosphate (G3P) to form lysophosphatidic acid (LPA). This enzyme utilizes acyl-phosphate as fatty acyl donor, but not acyl-CoA or acyl-ACP
Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP- GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5- monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain
Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template
K03628
-
-
2.453e-238
741.0
MMGS2_k127_2737374_1
Type VI secretion system, TssF
K11896
-
-
1.024e-235
743.0
MMGS2_k127_2737374_10
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaB. TsaE seems to play an indirect role in the t(6)A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD (By similarity)
DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function and is a proofreading 3'-5' exonuclease
Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)
K00791
-
2.5.1.75
0.000000000000000000007387
94.0
MMGS2_k127_2876752_3
Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction
Specifically methylates the N7 position of guanine in position 527 of 16S rRNA
K03501
-
2.1.1.170
0.00000000000000000000000000000000000000000001782
169.0
MMGS2_k127_29145_32
Outer membrane efflux protein
-
-
-
0.000000000000000000000000000000000000009742
164.0
MMGS2_k127_29145_34
Translation initiation factor 1A / IF-1
K02518
-
-
0.00000000000000000000000000000000002119
136.0
MMGS2_k127_29145_35
glyoxalase III activity
-
-
-
0.0000000000000000000000000000000002236
145.0
MMGS2_k127_29145_36
Uncharacterized conserved protein (DUF2267)
-
-
-
0.0000000000000000000000000000000008978
136.0
MMGS2_k127_29145_37
COG2009 Succinate dehydrogenase fumarate reductase, cytochrome b subunit
K00241
-
-
0.000000000000000000000000000000001421
133.0
MMGS2_k127_29145_38
Phosphoglycerate mutase family
-
-
-
0.000000000000000000000000000000002825
138.0
MMGS2_k127_29145_39
PFAM SOUL heme-binding protein
-
-
-
0.000000000000000000000000000000009139
130.0
MMGS2_k127_29145_4
Belongs to the FAD-dependent oxidoreductase 2 family. FRD SDH subfamily
K00239
-
1.3.5.1,1.3.5.4
3.203e-316
975.0
MMGS2_k127_29145_40
Hsp20/alpha crystallin family
K13993
-
-
0.0000000000000000000000000000006613
128.0
MMGS2_k127_29145_42
Ribonuclease E/G family
K08301
-
-
0.0000000000000000000000000006422
127.0
MMGS2_k127_29145_43
-
-
-
-
0.000000000000000000000000004355
120.0
MMGS2_k127_29145_44
-
-
-
-
0.0000000000000000000004217
105.0
MMGS2_k127_29145_45
succinate dehydrogenase
K00242
-
-
0.00000000000000000003194
96.0
MMGS2_k127_29145_46
DNA topoisomerase (ATP-hydrolyzing) inhibitor activity
K13652
-
-
0.000000000000000005292
86.0
MMGS2_k127_29145_47
Uncharacterized conserved protein (DUF2267)
-
-
-
0.0000000000000003757
84.0
MMGS2_k127_29145_48
sequence-specific DNA binding
-
-
-
0.00000000000003858
82.0
MMGS2_k127_29145_49
sequence-specific DNA binding
-
-
-
0.00000000495
66.0
MMGS2_k127_29145_5
Essential cell division protein that coordinates cell division and chromosome segregation. The N-terminus is involved in assembly of the cell-division machinery. The C-terminus functions as a DNA motor that moves dsDNA in an ATP-dependent manner towards the dif recombination site, which is located within the replication terminus region. Translocation stops specifically at Xer-dif sites, where FtsK interacts with the Xer recombinase, allowing activation of chromosome unlinking by recombination. FtsK orienting polar sequences (KOPS) guide the direction of DNA translocation. FtsK can remove proteins from DNA as it translocates, but translocation stops specifically at XerCD-dif site, thereby preventing removal of XerC and XerD from dif (By similarity)
K03466
-
-
2.959e-268
849.0
MMGS2_k127_29145_50
NAD FAD-binding protein
K06954
-
-
0.000000005667
58.0
MMGS2_k127_29145_52
-
-
-
-
0.0000001166
54.0
MMGS2_k127_29145_53
Transposase DDE domain
-
-
-
0.0000005819
55.0
MMGS2_k127_29145_54
-
-
-
-
0.00002414
55.0
MMGS2_k127_29145_55
Protein tyrosine kinase
K08884
-
2.7.11.1
0.00004261
56.0
MMGS2_k127_29145_56
Acetyltransferase (GNAT) domain
-
-
-
0.00005689
52.0
MMGS2_k127_29145_6
Belongs to the helicase family. UvrD subfamily
K16898
-
3.6.4.12
6.543e-232
754.0
MMGS2_k127_29145_7
ABC transporter transmembrane region
K12541
-
-
4.932e-198
640.0
MMGS2_k127_29145_8
Cell wall formation. Adds enolpyruvyl to UDP-N- acetylglucosamine
DNA-dependent ATPase and ATP-dependent 5'-3' DNA helicase. Has no activity on blunt DNA or DNA with 3'-overhangs, requires at least 10 bases of 5'-ssDNA for helicase activity
Provides the rickettsial cell with host ATP in exchange for rickettsial ADP. This is an obligate exchange system. This energy acquiring activity is an important component of rickettsial parasitism
COG0477 Permeases of the major facilitator superfamily
K05939
-
2.3.1.40,6.2.1.20
0.0
1375.0
MMGS2_k127_2967635_1
An AccC homodimer forms the biotin carboxylase subunit of the acetyl CoA carboxylase, an enzyme that catalyzes the formation of malonyl-CoA, which in turn controls the rate of fatty acid metabolism
first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA
amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile)
K01870
-
6.1.1.5
0.0
1358.0
MMGS2_k127_3009377_1
Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane
K07277
-
-
1.045e-310
968.0
MMGS2_k127_3009377_10
Bacterial Type VI secretion, VC_A0110, EvfL, ImpJ, VasE
NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient
Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched- chain and or straight-chain of fatty acids
Component of the acetyl coenzyme A carboxylase (ACC) complex. Biotin carboxylase (BC) catalyzes the carboxylation of biotin on its carrier protein (BCCP) and then the CO(2) group is transferred by the transcarboxylase to acetyl-CoA to form malonyl- CoA
Catalyzes the reversible formation of acyl-phosphate (acyl-PO(4)) from acyl- acyl-carrier-protein (acyl-ACP). This enzyme utilizes acyl-ACP as fatty acyl donor, but not acyl-CoA
Oxygenase that introduces the hydroxyl group at carbon five of 2-nonaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-nonaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol
COG0526 Thiol-disulfide isomerase and thioredoxins
-
-
-
0.0000000000000000000000000000000000000003387
156.0
MMGS2_k127_3009377_39
Diguanylate cyclase
-
-
-
0.0000000000000000000000000000000000000008133
168.0
MMGS2_k127_3009377_4
NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient (By similarity)
K00342
-
1.6.5.3
2.731e-222
700.0
MMGS2_k127_3009377_40
SmpA / OmlA family
-
-
-
0.00000000000000000000000000000000000005406
147.0
MMGS2_k127_3009377_41
GDP-mannose mannosyl hydrolase activity
-
-
-
0.0000000000000000000000000000000005595
135.0
MMGS2_k127_3009377_42
Bacterial DNA-binding protein
K05788
-
-
0.000000000000000000000000000000001226
131.0
MMGS2_k127_3009377_43
This protein is one of the two subunits of integration host factor, a specific DNA-binding protein that functions in genetic recombination as well as in transcriptional and translational control
K04764
-
-
0.000000000000000000000000005548
113.0
MMGS2_k127_3009377_44
Belongs to the bacterial ribosomal protein bL32 family
This protein is involved in the repair of mismatches in DNA. It is required for dam-dependent methyl-directed DNA mismatch repair. May act as a molecular matchmaker , a protein that promotes the formation of a stable complex between two or more DNA-binding proteins in an ATP-dependent manner without itself being part of a final effector complex
K03572
-
-
9.992e-213
676.0
MMGS2_k127_3009377_50
YnbE-like lipoprotein
-
-
-
0.0000000000001032
72.0
MMGS2_k127_3009377_51
Dicarboxylate transport
-
-
-
0.000000000003141
80.0
MMGS2_k127_3009377_52
COG2825 Outer membrane protein
-
-
-
0.00000001079
64.0
MMGS2_k127_3009377_53
Penicillin-Binding Protein C-terminus Family
K05367
-
2.4.1.129
0.00007062
45.0
MMGS2_k127_3009377_54
Protein of unknown function (DUF1318)
K09978
-
-
0.0002844
47.0
MMGS2_k127_3009377_6
ATPase that binds to both the 70S ribosome and the 50S ribosomal subunit in a nucleotide-independent manner
Catalyzes the synthesis of acetoacetyl coenzyme A from two molecules of acetyl coenzyme A. It can also act as a thiolase, catalyzing the reverse reaction and generating two-carbon units from the four-carbon product of fatty acid oxidation
Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan
Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc- (pentapeptide)GlcNAc (lipid intermediate II)
ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner
K01338
-
3.4.21.53
0.0
1152.0
MMGS2_k127_3095077_1
ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of ClpP
K03544
-
-
3.014e-232
722.0
MMGS2_k127_3095077_10
Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins
Forms passive diffusion pores that allow small molecular weight hydrophilic materials across the outer membrane
-
-
-
0.00000000000000000000000000000000000004024
161.0
MMGS2_k127_3095077_15
Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)
K00791
-
2.5.1.75
0.000000000000000000005131
94.0
MMGS2_k127_3095077_17
COG1961 Site-specific recombinases, DNA invertase Pin homologs
-
-
-
0.00000004088
55.0
MMGS2_k127_3095077_18
Belongs to the peptidase S8 family
-
-
-
0.000007332
58.0
MMGS2_k127_3095077_2
Belongs to the UDP-glucose GDP-mannose dehydrogenase family
Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bacterial cell walls
Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation. Functions as a peptidyl-prolyl cis-trans isomerase
An essential GTPase that binds both GDP and GTP, with rapid nucleotide exchange. Plays a role in 16S rRNA processing and 30S ribosomal subunit biogenesis and possibly also in cell cycle regulation and energy metabolism
Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre- crRNA and tracrRNA of type II CRISPR loci if present in the organism
damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage
K03702
-
-
0.0
1089.0
MMGS2_k127_3176306_1
2-Nitropropane dioxygenase
-
-
-
1.229e-260
808.0
MMGS2_k127_3176306_2
Involved in transcription antitermination. Required for transcription of ribosomal RNA (rRNA) genes. Binds specifically to the boxA antiterminator sequence of the ribosomal RNA (rrn) operons
K03625
-
-
0.0000000000000000000000000000000000007801
144.0
MMGS2_k127_3176306_3
Protein required for attachment to host cells
-
-
-
0.0000000000000000009509
93.0
MMGS2_k127_3176306_5
-
-
-
-
0.000005398
56.0
MMGS2_k127_3269928_0
The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by UvrB, the UvrA molecules dissociate
Participates in the translocation of lipoproteins from the inner membrane to the outer membrane. Only forms a complex with a lipoprotein if the residue after the N-terminal Cys is not an aspartate (The Asp acts as a targeting signal to indicate that the lipoprotein should stay in the inner membrane)
-
-
-
0.000000000000000000000000000000000000002313
154.0
MMGS2_k127_3518551_9
NUDIX domain
-
-
-
0.00000000000000000000000000000000005643
142.0
MMGS2_k127_3605519_0
Negative regulator of class I heat shock genes (grpE- dnaK-dnaJ and groELS operons). Prevents heat-shock induction of these operons
Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates
Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits)
Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA RNA and avoiding chromosomal lesions
Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule
Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit
Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase
thus facilitating recognition of the initiation point. It is needed to translate mRNA with a short Shine-Dalgarno (SD) purine-rich sequence
K02945
-
-
2.804e-292
908.0
MMGS2_k127_3776766_1
Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)
Accelerates the degradation of transcripts by removing pyrophosphate from the 5'-end of triphosphorylated RNA, leading to a more labile monophosphorylated state that can stimulate subsequent ribonuclease cleavage
Part of the ABC transporter complex LolCDE involved in the translocation of mature outer membrane-directed lipoproteins, from the inner membrane to the periplasmic chaperone, LolA. Responsible for the formation of the LolA-lipoprotein complex in an ATP-dependent manner
Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone
K03168
-
5.99.1.2
1.776e-306
953.0
MMGS2_k127_3991889_0
Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate
Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate
Belongs to the CDP-alcohol phosphatidyltransferase class-I family
K00995,K08744
-
2.7.8.41,2.7.8.5
0.00000000000000000000000002094
116.0
MMGS2_k127_4282484_9
Sugar efflux transporter for intercellular exchange
K15383
-
-
0.00000001724
59.0
MMGS2_k127_4360498_0
Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and or for immediate growth after restoration of oxygen
Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp Asn)
NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. This subunit may bind ubiquinone
Catalyzes the interconversion between ADP-D-glycero- beta-D-manno-heptose and ADP-L-glycero-beta-D-manno-heptose via an epimerization at carbon 6 of the heptose
NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain
K00335
-
1.6.5.3
3.7e-230
716.0
MMGS2_k127_4433546_20
Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient
NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient
NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient
NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient (By similarity)
NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient
This protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site
An accessory protein needed during the final step in the assembly of 30S ribosomal subunit, possibly for assembly of the head region. Probably interacts with S19. Essential for efficient processing of 16S rRNA. May be needed both before and after RbfA during the maturation of 16S rRNA. It has affinity for free ribosomal 30S subunits but not for 70S ribosomes
K02860
-
-
0.00000000000000000000000000000000000000001585
160.0
MMGS2_k127_4433546_34
Belongs to the bacterial ribosomal protein bS16 family
Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions
K03530
-
-
0.00000000000000000000000000000000000003752
144.0
MMGS2_k127_4433546_36
NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient
NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient
K00333
-
1.6.5.3
8.929e-219
682.0
MMGS2_k127_4433546_40
GIY-YIG catalytic domain
K07461
-
-
0.000000000000000002151
86.0
MMGS2_k127_4433546_5
Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY. Interaction with FtsY leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components
Critical role in recombination and DNA repair. Helps process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3'- to 5'- polarity. Unwinds branched duplex DNA (Y-DNA)
K03655
-
3.6.4.12
4.634e-238
754.0
MMGS2_k127_4499876_1
Component of the acetyl coenzyme A carboxylase (ACC) complex. First, biotin carboxylase catalyzes the carboxylation of biotin on its carrier protein (BCCP) and then the CO(2) group is transferred by the carboxyltransferase to acetyl-CoA to form malonyl-CoA
Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides
Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone
The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB
The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing
Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis
K00412
-
-
9.814e-224
697.0
MMGS2_k127_5108117_20
oxidoreductase activity, acting on CH-OH group of donors
Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis
Belongs to the bacterial ribosomal protein bL28 family
K02902
-
-
0.000000000000000000000000000000004431
130.0
MMGS2_k127_5108117_33
2-phosphosulpholactate phosphatase
K05979
-
3.1.3.71
0.00000000000000000000000000000002233
137.0
MMGS2_k127_5108117_34
Protein of unknown function (DUF721)
-
-
-
0.00000000000000000000000000003952
122.0
MMGS2_k127_5108117_35
sequence-specific DNA binding
-
-
-
0.00000000000000000000000004793
114.0
MMGS2_k127_5108117_36
von Willebrand factor (vWF) type A domain
-
-
-
0.0000000000000000000000009914
119.0
MMGS2_k127_5108117_37
transferase activity, transferring glycosyl groups
K13677
-
2.4.1.208
0.000000000000000000000157
110.0
MMGS2_k127_5108117_38
-
-
-
-
0.0000000000000004423
86.0
MMGS2_k127_5108117_39
Zinc-finger domain
-
-
-
0.0000000005847
61.0
MMGS2_k127_5108117_4
COG1132 ABC-type multidrug transport system, ATPase and permease components
K06147
-
-
1.415e-204
652.0
MMGS2_k127_5108117_40
Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8- dihydroguanosine 5'-triphosphate
K03639
-
4.1.99.22
0.000000001409
69.0
MMGS2_k127_5108117_41
GTPase activator activity
-
-
-
0.00000003327
58.0
MMGS2_k127_5108117_42
COG1961 Site-specific recombinases, DNA invertase Pin homologs
-
-
-
0.0000000817
54.0
MMGS2_k127_5108117_43
Psort location OuterMembrane, score 9.49
-
-
-
0.00005539
55.0
MMGS2_k127_5108117_44
-
-
-
-
0.0004302
48.0
MMGS2_k127_5108117_5
Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2- cyclopenten-1-yl)amino)methyl)-7-deazaguanosine)
An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. Plays a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control
Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine
IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit
IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to HisF for the synthesis of IGP and AICAR
Single strand-specific metallo-endoribonuclease involved in late-stage 70S ribosome quality control and in maturation of the 3' terminus of the 16S rRNA
K07042
-
-
0.000000000000000000000000000000000000000000673
162.0
MMGS2_k127_5241354_29
Type II secretion system (T2SS), protein F
K02653
-
-
0.00000000000000000000000000000000000000001329
168.0
MMGS2_k127_5241354_3
Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA
Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth
K03086
-
-
1.547e-268
840.0
MMGS2_k127_5328092_1
RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication
This enzyme is involved in nucleotide metabolism it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA
F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation
Methyltransferase required for the conversion of demethylmenaquinol (DMKH2) to menaquinol (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2- polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2)
Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate
Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity
This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly
Belongs to the bacterial ribosomal protein bL33 family
K02913
-
-
0.000000000000000000000000006593
111.0
MMGS2_k127_5436345_5
Autotransporter beta-domain
-
-
-
0.000000000000000003401
97.0
MMGS2_k127_5436345_6
-O-antigen
-
-
-
0.0004357
52.0
MMGS2_k127_5518232_0
amino acids such as threonine, to avoid such errors, it has a posttransfer editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner
K01873
-
6.1.1.9
0.0
1232.0
MMGS2_k127_5518232_1
DNA helicase
K03657
-
3.6.4.12
1.1e-272
859.0
MMGS2_k127_5518232_2
DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity
Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules. The XerC-XerD complex is essential to convert dimers of the bacterial chromosome into monomers to permit their segregation at cell division. It also contributes to the segregational stability of plasmids
Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP
Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines
Catalyzes the reductive methylation of 2'-deoxyuridine- 5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor, and NADPH and FADH(2) as the reductant
One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex
K02519
-
-
0.0
1020.0
MMGS2_k127_5613356_1
Produces ATP from ADP in the presence of a proton gradient across the membrane. The catalytic sites are hosted primarily by the beta subunits
K02112
-
3.6.3.14
1.464e-259
805.0
MMGS2_k127_5613356_10
Involved in peptidoglycan biosynthesis. Transports lipid-linked peptidoglycan precursors from the inner to the outer leaflet of the cytoplasmic membrane
Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (IspD), and catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4- cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP) (IspF)
DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N- glycosidic bond, leaving an AP (apurinic apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
Produces ATP from ADP in the presence of a proton gradient across the membrane. The alpha chain is a regulatory subunit
K02111
-
3.6.3.14
4.591e-252
785.0
MMGS2_k127_5613356_20
Redox regulated molecular chaperone. Protects both thermally unfolding and oxidatively damaged proteins from irreversible aggregation. Plays an important role in the bacterial defense system toward oxidative stress
Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex
Participates in both transcription termination and antitermination
K02600
-
-
4.385e-227
713.0
MMGS2_k127_5613356_30
Transglycosylase SLT domain
-
-
-
0.0000000000000000000000000000000000005004
147.0
MMGS2_k127_5613356_31
F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation
K02113
-
-
0.0000000000000000000000000000003373
130.0
MMGS2_k127_5613356_32
Sel1-like repeats.
-
-
-
0.0000000000000000000000000001477
124.0
MMGS2_k127_5613356_34
DUF218 domain
-
-
-
0.00000000000000000000000005451
115.0
MMGS2_k127_5613356_35
One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Required for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA
K02834
-
-
0.000000000000000000000001001
109.0
MMGS2_k127_5613356_36
Produces ATP from ADP in the presence of a proton gradient across the membrane
K02114
-
-
0.000000000000000000004341
95.0
MMGS2_k127_5613356_37
Mediates coordination of peptidoglycan synthesis and outer membrane constriction during cell division
-
-
-
0.000000000000000004965
96.0
MMGS2_k127_5613356_38
Major Facilitator
-
-
-
0.000000000000004717
78.0
MMGS2_k127_5613356_39
FOG TPR repeat
-
-
-
0.000000000006767
75.0
MMGS2_k127_5613356_4
PD-(D/E)XK nuclease superfamily
K16899
-
3.6.4.12
1.24e-204
670.0
MMGS2_k127_5613356_40
phage envelope protein
-
-
-
0.000000009354
59.0
MMGS2_k127_5613356_41
Belongs to the small heat shock protein (HSP20) family
K13993
-
-
0.000001624
58.0
MMGS2_k127_5613356_5
Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)
K01885
-
6.1.1.17
1.192e-201
636.0
MMGS2_k127_5613356_6
Poly(3-hydroxyalkanoate) synthetase
K03821
-
-
3.355e-198
638.0
MMGS2_k127_5613356_7
Belongs to the class-I aminoacyl-tRNA synthetase family
Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P)
Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions
RNA chaperone that binds small regulatory RNA (sRNAs) and mRNAs to facilitate mRNA translational regulation in response to envelope stress, environmental stress and changes in metabolite concentrations. Also binds with high specificity to tRNAs
K03666
-
-
0.00000000000000000000000000000000000000000007939
160.0
MMGS2_k127_567898_24
YbaK prolyl-tRNA synthetase
K19055
-
-
0.0000000000000000000000000000000000004332
145.0
MMGS2_k127_567898_26
-
-
-
-
0.0000000000000000000000000000453
130.0
MMGS2_k127_567898_27
BON domain
-
-
-
0.00000000000000000000000004584
111.0
MMGS2_k127_567898_28
ChaB
K06197
-
-
0.000000000000000000000004708
104.0
MMGS2_k127_567898_29
Uncharacterized protein conserved in bacteria (DUF2312)
-
-
-
0.000000000000000000000008473
103.0
MMGS2_k127_567898_3
Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs
K08300,K08301
-
3.1.26.12
4.758e-211
682.0
MMGS2_k127_567898_30
-
-
-
-
0.0000000000000000009353
99.0
MMGS2_k127_567898_32
light absorption
-
-
-
0.000000000005034
73.0
MMGS2_k127_567898_33
Belongs to the peptidase S1C family
K04771,K04772
-
3.4.21.107
0.00004244
52.0
MMGS2_k127_567898_4
aminopeptidase
K01262
-
3.4.11.9
3.624e-207
662.0
MMGS2_k127_567898_5
GTPase that associates with the 50S ribosomal subunit and may have a role during protein synthesis or ribosome biogenesis
Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)- butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP MEP pathway for isoprenoid precursor biosynthesis
The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently
K03076
-
-
3.601e-221
692.0
MMGS2_k127_5684037_1
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates
One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome
This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7 L12 stalk, and near the tRNA binding site of the peptidyltransferase center
Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine-Dalgarno cleft in the 70S ribosome
One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity
Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits
Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism
One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit
its binding is stimulated by other ribosomal proteins, e.g. L4, L17, and L20. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome (By similarity)
K02890
-
-
0.00000000000000000000000000000000000000001317
157.0
MMGS2_k127_5684037_28
This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance
K02881
-
-
0.0000000000000000000000000000000000000003891
151.0
MMGS2_k127_5684037_29
One of two assembly initiator proteins, it binds directly to the 5'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit
One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA
K02961
-
-
0.000000000000000000000000000001378
122.0
MMGS2_k127_5684037_35
One of the early assembly proteins it binds 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome. Forms the main docking site for trigger factor binding to the ribosome
This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits
Required for the insertion and or proper folding and or complex formation of integral membrane proteins into the membrane. Involved in integration of membrane proteins that insert both dependently and independently of the Sec translocase complex, as well as at least some lipoproteins. Aids folding of multispanning membrane proteins
K03217
-
-
1.874e-244
766.0
MMGS2_k127_5684469_10
Required for the activity of the bacterial periplasmic transport system of putrescine
Belongs to the transferase hexapeptide repeat family
K00674
-
2.3.1.117
0.0000000000000000000000000000000003968
137.0
MMGS2_k127_5684469_22
negative regulation of transcription, DNA-templated
K21600
-
-
0.00000000000000000000000000000004175
127.0
MMGS2_k127_5684469_23
Disulfide bond formation protein, DsbB
K03611
-
-
0.0000000000000000000000000000003831
128.0
MMGS2_k127_5684469_24
COG0583 Transcriptional regulator
-
-
-
0.00000000000000000000000000001861
129.0
MMGS2_k127_5684469_25
mercury ion transmembrane transporter activity
-
-
-
0.000000000000000000000000000967
119.0
MMGS2_k127_5684469_27
Could be involved in insertion of integral membrane proteins into the membrane
K08998
-
-
0.000000000000000000000000002071
114.0
MMGS2_k127_5684469_28
RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme
Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit
Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit
Non-ribosomal peptide synthetase modules and related proteins
K13611,K13614,K15654
-
-
0.00000000000000000000000000000001206
137.0
MMGS2_k127_621886_7
-
-
-
-
0.0000000000000001015
89.0
MMGS2_k127_621886_8
DEAD-like helicases superfamily
-
-
-
0.00000000000004698
74.0
MMGS2_k127_621886_9
Transcriptional regulator
-
-
-
0.00000000000005451
74.0
MMGS2_k127_636015_0
Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving both as a receptor for the preprotein-SecB complex and as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane
K03070
-
-
0.0
1286.0
MMGS2_k127_636015_1
Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family
Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC). Interaction with SRP-RNC leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components
Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA
Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. Together with TatB, TatC is part of a receptor directly interacting with Tat signal peptides
Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates
this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N-terminal part of its protein substrates and unfolds these before they are guided to HslV for hydrolysis
One of the proteins required for the normal export of preproteins out of the cell cytoplasm. It is a molecular chaperone that binds to a subset of precursor proteins, maintaining them in a translocation-competent state. It also specifically binds to its receptor SecA
ATP-dependent protease La (LON) substrate-binding domain
K07157
-
-
0.00000000000000000000000000000000000000000006488
168.0
MMGS2_k127_636015_39
-
-
-
-
0.000000000000000000000000000000000000001334
154.0
MMGS2_k127_636015_4
Uncharacterized protein family UPF0004
K18707
-
2.8.4.5
8.875e-199
629.0
MMGS2_k127_636015_40
Hemerythrin HHE cation binding domain
-
-
-
0.000000000000000000000000000000000000006482
150.0
MMGS2_k127_636015_41
Lysylphosphatidylglycerol synthase TM region
K07027
-
-
0.0000000000000000000000000000000002386
144.0
MMGS2_k127_636015_42
The SecYEG-SecDF-YajC-YidC holo-translocon (HTL) protein secretase insertase is a supercomplex required for protein secretion, insertion of proteins into membranes, and assembly of membrane protein complexes. While the SecYEG complex is essential for assembly of a number of proteins and complexes, the SecDF- YajC-YidC subcomplex facilitates these functions
K03210
-
-
0.000000000000000000000000000002161
124.0
MMGS2_k127_636015_43
Uroporphyrinogen-III synthase HemD
K01719
-
4.2.1.75
0.0000000000000000000000000004954
122.0
MMGS2_k127_636015_44
Smr domain
-
-
-
0.000000000000000000000000003837
118.0
MMGS2_k127_636015_45
C-terminal domain of 1-Cys peroxiredoxin
K03386
-
1.11.1.15
0.0000000000000000000000006103
106.0
MMGS2_k127_636015_46
Protein required for attachment to host cells
-
-
-
0.00000000000000000000008093
104.0
MMGS2_k127_636015_47
Low affinity iron permease
-
-
-
0.000000000000000000001012
99.0
MMGS2_k127_636015_48
Belongs to the UPF0434 family
K09791
-
-
0.00000000000000000004218
91.0
MMGS2_k127_636015_5
Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec)
Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. TatA could form the protein-conducting channel of the Tat system
K03116
-
-
0.0000000000000167
75.0
MMGS2_k127_636015_51
-
-
-
-
0.0000000000005874
79.0
MMGS2_k127_636015_52
Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. Together with TatC, TatB is part of a receptor directly interacting with Tat signal peptides. TatB may form an oligomeric binding site that transiently accommodates folded Tat precursor proteins before their translocation
Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA
DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase (By similarity)
K02337
-
2.7.7.7
0.0
1462.0
MMGS2_k127_67051_1
COG4799 Acetyl-CoA carboxylase, carboxyltransferase component (subunits alpha and beta)
K01966
-
2.1.3.15,6.4.1.3
5.568e-267
829.0
MMGS2_k127_67051_10
Metal-dependent hydrolases of the beta-lactamase superfamily I
Catalyzes the stereoinversion of LL-2,6- diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso- DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan
Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation
K01874
-
6.1.1.10
4.397e-231
724.0
MMGS2_k127_67051_20
Involved in unsaturated fatty acids biosynthesis. Catalyzes the dehydration of short chain beta-hydroxyacyl-ACPs and long chain saturated and unsaturated beta-hydroxyacyl-ACPs
Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell
Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln)
K02434
-
6.3.5.6,6.3.5.7
1.311e-252
786.0
MMGS2_k127_786133_1
Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln)
Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln)
Couples transcription and DNA repair by recognizing RNA polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent release of RNAP and its truncated transcript from the DNA, and recruitment of nucleotide excision repair machinery to the damaged site
K03723
-
-
0.0
1164.0
MMGS2_k127_827059_10
COG1249 Pyruvate 2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes
In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance
Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine
Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA
K00783
-
2.1.1.177
0.00000000000000000000000000000000000000000001008
166.0
MMGS2_k127_827059_45
COG2927 DNA polymerase III, chi subunit
K02339
-
2.7.7.7
0.000000000000000000000000000000000000000001688
160.0
MMGS2_k127_827059_46
Conserved hypothetical protein 95
K08316
-
2.1.1.171
0.000000000000000000000000000000000000000009022
160.0
MMGS2_k127_827059_47
Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of acyl-carrier-protein
K00997
-
2.7.8.7
0.00000000000000000000000000000000000000002074
156.0
MMGS2_k127_827059_48
SelR domain
K07305
-
1.8.4.12
0.0000000000000000000000000000000000000002667
156.0
MMGS2_k127_827059_49
ABC-type transport auxiliary lipoprotein component
K18480
-
-
0.0000000000000000000000000000000000000003738
157.0
MMGS2_k127_827059_5
HipA N-terminal domain
K07154
-
2.7.11.1
6.594e-200
630.0
MMGS2_k127_827059_50
Functions as a ribosomal silencing factor. Interacts with ribosomal protein L14 (rplN), blocking formation of intersubunit bridge B8. Prevents association of the 30S and 50S ribosomal subunits and the formation of functional ribosomes, thus repressing translation
K09710
-
-
0.000000000000000000000000000000000000007006
148.0
MMGS2_k127_827059_51
Promotes RNA polymerase assembly. Latches the N- and C- terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits
K03060
-
2.7.7.6
0.00000000000000000000000000000000000007849
146.0
MMGS2_k127_827059_52
Cupin 2, conserved barrel domain protein
K11312
-
-
0.0000000000000000000000000000000000013
142.0
MMGS2_k127_827059_53
COGs COG3677 Transposase and inactivated derivatives
K07480
-
-
0.00000000000000000000000000000000003384
138.0
MMGS2_k127_827059_54
Binds the 23S rRNA
K02909
-
-
0.00000000000000000000000000000000004616
135.0
MMGS2_k127_827059_55
PFAM Cytochrome b(N-terminal) b6 petB
K12262
-
-
0.0000000000000000000000000000000001373
139.0
MMGS2_k127_827059_56
ribosomal protein S21
K02970
-
-
0.000000000000000000000000001494
112.0
MMGS2_k127_827059_57
Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins
K03676
-
-
0.000000000000000000000000205
107.0
MMGS2_k127_827059_58
Lipopolysaccharide-assembly, LptC-related
K11719
-
-
0.000000000000000000000000229
115.0
MMGS2_k127_827059_6
Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides
Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins
Involved in the gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) through direct phosphoryl transfer between the nucleoside triphosphate and OAA
Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr)
K01868
-
6.1.1.3
2.508e-319
986.0
MMGS2_k127_827424_10
Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family
Exonuclease involved in the 3' processing of various precursor tRNAs. Initiates hydrolysis at the 3'-terminus of an RNA molecule and releases 5'-mononucleotides
Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration
Transcription factor that acts by binding directly to the RNA polymerase (RNAP). Required for negative regulation of rRNA expression and positive regulation of several amino acid biosynthesis promoters
Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ
to multidrug resistance ABC transporter ATP-binding protein
K06147
-
-
7.824e-265
826.0
MMGS2_k127_827424_20
This protein binds to 23S rRNA in the presence of protein L20
K02888
-
-
0.0000000000000000000000000000000000000002073
153.0
MMGS2_k127_827424_21
Belongs to the bacterial ribosomal protein bL27 family
K02899
-
-
0.0000000000000000000000000000000000000303
144.0
MMGS2_k127_827424_22
response to heat
K07090
-
-
0.00000000000000000000000000000000000005553
152.0
MMGS2_k127_827424_23
colicin V production
K03558
-
-
0.0000000000000000000000000000000000009446
151.0
MMGS2_k127_827424_24
PFAM RNA recognition motif
-
-
-
0.0000000000000000000000000000002958
126.0
MMGS2_k127_827424_25
Part of the high-affinity ATP-driven potassium transport (or Kdp) system, which catalyzes the hydrolysis of ATP coupled with the electrogenic transport of potassium into the cytoplasm. This subunit acts as a catalytic chaperone that increases the ATP- binding affinity of the ATP-hydrolyzing subunit KdpB by the formation of a transient KdpB KdpC ATP ternary complex
DNA-dependent ATPase involved in processing of recombination intermediates, plays a role in repairing DNA breaks. Stimulates the branch migration of RecA-mediated strand transfer reactions, allowing the 3' invading strand to extend heteroduplex DNA faster. Binds ssDNA in the presence of ADP but not other nucleotides, has ATPase activity that is stimulated by ssDNA and various branched DNA structures, but inhibited by SSB. Does not have RecA's homology-searching function
ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins
Part of the high-affinity ATP-driven potassium transport (or Kdp) system, which catalyzes the hydrolysis of ATP coupled with the electrogenic transport of potassium into the cytoplasm. This subunit binds and transports the potassium across the cytoplasmic membrane
