Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits
K02528
-
2.1.1.182
0.000000000000000000000000000000000000007632
155.0
MMS1_k127_1120565_4
Belongs to the UPF0200 family
-
-
-
0.000000000000000000000003103
109.0
MMS1_k127_1120565_5
Diguanylate cyclase, GGDEF domain
-
-
-
0.00000000000000000000007975
111.0
MMS1_k127_1120565_6
Large-conductance mechanosensitive channel, MscL
K03282
-
-
0.0000000000000000000001643
100.0
MMS1_k127_1120565_7
ester cyclase
-
-
-
0.000000000000000000008414
95.0
MMS1_k127_1120565_8
Heavy metal translocating P-type atpase
-
-
-
0.0000000000000000000258
100.0
MMS1_k127_1120565_9
amine dehydrogenase activity
-
-
-
0.0000000000000000006724
101.0
MMS1_k127_1229198_0
Produces ATP from ADP in the presence of a proton gradient across the membrane. The archaeal alpha chain is a catalytic subunit
Catalyzes the irreversible transfer of a propylamine group from the amino donor S-adenosylmethioninamine (decarboxy- AdoMet) to putrescine (1,4-diaminobutane) to yield spermidine
Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine
tRNA-splicing ligase that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs
Prenyltransferase that catalyzes the transfer of the geranylgeranyl moiety of geranylgeranyl diphosphate (GGPP) to the C2 hydroxyl of (S)-3-O-geranylgeranylglyceryl phosphate (GGGP). This reaction is the second ether-bond-formation step in the biosynthesis of archaeal membrane lipids
K17105
-
2.5.1.42
0.0000000000000000000000000000000000002604
153.0
MMS1_k127_1254652_4
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates
This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly
K02871
-
-
0.00000000000000000000000000001518
123.0
MMS1_k127_1254652_6
Belongs to the universal ribosomal protein uS9 family
K02996
-
-
0.0000000000000000000000000005649
119.0
MMS1_k127_1254652_7
UbiA prenyltransferase family
K02548
-
2.5.1.74
0.00000000000000000000000001004
123.0
MMS1_k127_1254652_8
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates
transferase activity, transferring acyl groups other than amino-acyl groups
-
-
-
0.0001841
53.0
MMS1_k127_1372008_2
Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration
Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and or degradation of damaged proteins
Binds double-stranded DNA tightly but without sequence specificity. It is distributed uniformly and abundantly on the chromosome, suggesting a role in chromatin architecture. However, it does not significantly compact DNA. Binds rRNA and mRNA in vivo. May play a role in maintaining the structural and functional stability of RNA, and, perhaps, ribosomes
K03622
-
-
0.00000000000000000000000002574
113.0
MMS1_k127_142742_7
membrane
-
-
-
0.0000000001522
73.0
MMS1_k127_142742_8
-
-
-
-
0.0000000004727
63.0
MMS1_k127_142742_9
helix_turn_helix ASNC type
-
-
-
0.00003137
55.0
MMS1_k127_1538810_0
Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome
K03234
-
-
1.663e-239
761.0
MMS1_k127_1538810_1
Belongs to the peptidase S16 family
K04076
-
3.4.21.53
7.983e-204
653.0
MMS1_k127_1538810_10
Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY
Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity
Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Kae1 likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity
Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain
The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently
Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC)
Catalytic component of the exosome, which is a complex involved in RNA degradation. Has 3'- 5' exoribonuclease activity. Can also synthesize heteropolymeric RNA-tails
NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient
One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center
May function in recognizing stalled ribosomes, interact with stem-loop structures in stalled mRNA molecules, and effect endonucleolytic cleavage of the mRNA. May play a role in the release non-functional ribosomes and degradation of damaged mRNAs. Has endoribonuclease activity
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function
Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, the RNA component of RNase P, box H ACA, box C D and box C' D' sRNAs
Catalyzes the formation of CDP-2,3-bis-(O- geranylgeranyl)-sn-glycerol (CDP-archaeol) from 2,3-bis-(O- geranylgeranyl)-sn-glycerol 1-phosphate (DGGGP) and CTP. This reaction is the third ether-bond-formation step in the biosynthesis of archaeal membrane lipids
K19664
-
2.7.7.67
0.000000000000000000000000000001136
128.0
MMS1_k127_1538810_57
TatD related DNase
K03424
-
-
0.000000000000000000000000000002733
130.0
MMS1_k127_1538810_58
Non-catalytic component of the exosome, which is a complex involved in RNA degradation. Increases the RNA binding and the efficiency of RNA degradation. Confers strong poly(A) specificity to the exosome
NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient
Belongs to the eukaryotic ribosomal protein eL30 family
K02908
-
-
0.000000000000000002486
88.0
MMS1_k127_1538810_66
General factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Binds specifically to the TATA box promoter element which lies close to the position of transcription initiation
K03120
-
-
0.0000000000000001031
85.0
MMS1_k127_1538810_67
Belongs to the eukaryotic ribosomal protein eS28 family
K02979
-
-
0.0000000000000006124
79.0
MMS1_k127_1538810_68
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates
K03055
-
2.7.7.6
0.000000000000001053
79.0
MMS1_k127_1538810_69
binds to the 23S rRNA
K02921
-
-
0.000000000000002187
78.0
MMS1_k127_1538810_7
Is involved in the reduction of 2,3- digeranylgeranylglycerophospholipids (unsaturated archaeols) into 2,3-diphytanylglycerophospholipids (saturated archaeols) in the biosynthesis of archaeal membrane lipids. Catalyzes the formation of archaetidic acid (2,3-di-O-phytanyl-sn-glyceryl phosphate) from 2,3-di-O-geranylgeranylglyceryl phosphate (DGGGP) via the hydrogenation of each double bond of the isoprenoid chains
PFAM Haloacid dehalogenase domain protein hydrolase
-
-
-
0.0000000000242
72.0
MMS1_k127_1538810_72
peptidase
-
-
-
0.0000000001416
67.0
MMS1_k127_1538810_73
Domain of unknown function (DUF929)
-
-
-
0.0000000003623
71.0
MMS1_k127_1538810_74
-
-
-
-
0.0000001102
58.0
MMS1_k127_1538810_75
Molecular chaperone capable of stabilizing a range of proteins. Seems to fulfill an ATP-independent, HSP70-like function in archaeal de novo protein folding
Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate
General factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Binds specifically to the TATA box promoter element which lies close to the position of transcription initiation
K03120
-
-
0.0000000000000000008304
92.0
MMS1_k127_174266_11
Na -transporting NADH ubiquinone oxidoreductase subunit
-
-
-
0.000000215
61.0
MMS1_k127_174266_12
Belongs to the eukaryotic ribosomal protein eL21 family
DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N- glycosidic bond, leaving an AP (apurinic apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N- glycosidic bond, leaving an AP (apurinic apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. Also part of the exosome, which is a complex involved in RNA degradation. Acts as a poly(A)-binding protein that enhances the interaction between heteropolymeric, adenine-rich transcripts and the exosome
Prenyltransferase that catalyzes the transfer of the geranylgeranyl moiety of geranylgeranyl diphosphate (GGPP) to the C3 hydroxyl of sn-glycerol-1-phosphate (G1P). This reaction is the first ether-bond-formation step in the biosynthesis of archaeal membrane lipids
Zinc phosphodiesterase, which displays some tRNA 3'- processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA
Catalytic subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. The small subunit contains the primase catalytic core and has DNA synthesis activity on its own. Binding to the large subunit stabilizes and modulates the activity, increasing the rate of DNA synthesis while decreasing the length of the DNA fragments, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair
One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit
This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance
This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits
This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7 L12 stalk, and near the tRNA binding site of the peptidyltransferase center
K02933
-
-
0.000000000000000000000000000006176
125.0
MMS1_k127_2258272_27
Transcription elongation factor Spt5
K02601
-
-
0.0000000000000000000000000001015
121.0
MMS1_k127_2258272_28
One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA
Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome
K02874
-
-
0.00000000000000000000000000176
116.0
MMS1_k127_2258272_3
Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec)
6-O-methylguanine DNA methyltransferase, DNA binding domain
K00567,K10778
-
2.1.1.63
0.0000000000000001641
83.0
MMS1_k127_2258272_36
One of two assembly initiator proteins, it binds directly to the 5'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit
K02895
-
-
0.000000000000009274
84.0
MMS1_k127_2258272_37
AAA ATPase
-
-
-
0.00000000000004723
85.0
MMS1_k127_2258272_38
Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends
K03538
-
3.1.26.5
0.000000000001045
72.0
MMS1_k127_2258272_39
Activates the tRNA-splicing ligase complex by facilitating the enzymatic turnover of catalytic subunit RtcB. Acts by promoting the guanylylation of RtcB, a key intermediate step in tRNA ligation. Can also alter the NTP specificity of RtcB such that ATP, dGTP or ITP is used efficiently
-
-
-
0.000000000001077
74.0
MMS1_k127_2258272_4
Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr)
Non-catalytic component of the exosome, which is a complex involved in RNA degradation. Increases the RNA binding and the efficiency of RNA degradation. Helpful for the interaction of the exosome with A-poor RNAs
K07573
-
-
0.00000000003893
72.0
MMS1_k127_2258272_43
ParE toxin of type II toxin-antitoxin system, parDE
K06218
-
-
0.00000006821
57.0
MMS1_k127_2258272_44
Participates in chromosomal partition during cell division. May act via the formation of a condensin-like complex containing Smc and ScpA that pull DNA away from mid-cell into both cell halves
K06024
-
-
0.000001784
56.0
MMS1_k127_2258272_45
small metal-binding protein
-
-
-
0.000006007
51.0
MMS1_k127_2258272_46
pathogenesis
-
-
-
0.00002937
57.0
MMS1_k127_2258272_47
Essential subunit of the Sec protein translocation channel SecYEG. Clamps together the 2 halves of SecY. May contact the channel plug during translocation
Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp Asn)
A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination. Cleaves 4-way DNA junctions introducing paired nicks in opposing strands, leaving a 5'-terminal phosphate and a 3'-terminal hydroxyl group that are ligated to produce recombinant products
K03552
-
3.1.22.4
0.000001891
55.0
MMS1_k127_2576795_0
Mediates influx of magnesium ions
K03284
-
-
0.00000000000000000000000000000000000000005365
164.0
MMS1_k127_2576795_1
Leucine rich repeat protein
-
-
-
0.00000000000000000000000001265
126.0
MMS1_k127_2576795_2
Winged helix DNA-binding domain
-
-
-
0.000000000000000005738
87.0
MMS1_k127_2576795_3
regulation of response to stimulus
-
-
-
0.00001254
59.0
MMS1_k127_2633096_0
-
-
-
-
0.00000000002726
66.0
MMS1_k127_2633096_1
Belongs to the eukaryotic ribosomal protein eL40 family
K02927
-
-
0.000000006681
57.0
MMS1_k127_2692830_0
Carbamoyl-phosphate synthetase large chain, oligomerisation
Possesses two activities a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase
K02322
-
2.7.7.7
4.507e-287
919.0
MMS1_k127_2692830_10
Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr)
Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors
K02869
-
-
0.000000000000000000169
91.0
MMS1_k127_2692830_102
PFAM conserved
-
-
-
0.000000000000000002032
97.0
MMS1_k127_2692830_103
sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp
K01170
-
4.6.1.16
0.000000000000000003444
97.0
MMS1_k127_2692830_104
membrane
-
-
-
0.00000000000000004718
96.0
MMS1_k127_2692830_105
Nucleotidyl transferase AbiEii toxin, Type IV TA system
K09144
-
-
0.0000000000000001618
87.0
MMS1_k127_2692830_106
phosphatase activity
K07025
-
-
0.0000000000000003168
86.0
MMS1_k127_2692830_107
Uncharacterized ACR, COG1430
K09005
-
-
0.000000000000001054
81.0
MMS1_k127_2692830_108
PFAM helix-turn-helix domain protein
K03627
-
-
0.000000000000001735
82.0
MMS1_k127_2692830_109
Hydrolyzes RNA 2',3'-cyclic phosphodiester to an RNA 2'- phosphomonoester
K01975
-
3.1.4.58
0.000000000000008621
81.0
MMS1_k127_2692830_11
Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)
The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome
K02890
-
-
0.000000000001276
76.0
MMS1_k127_2692830_116
Belongs to the small heat shock protein (HSP20) family
K13993
-
-
0.000000000001416
74.0
MMS1_k127_2692830_117
Belongs to the cytidylate kinase family. Type 2 subfamily
K00945
-
2.7.4.25
0.00000000000142
75.0
MMS1_k127_2692830_118
-
-
-
-
0.000000000001544
71.0
MMS1_k127_2692830_119
binds to the 23S rRNA
K02929
-
-
0.000000000002419
70.0
MMS1_k127_2692830_12
Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and or for immediate growth after restoration of oxygen
Transcription factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Facilitates transcription initiation by enhancing TATA-box recognition by TATA-box-binding protein (Tbp), and transcription factor B (Tfb) and RNA polymerase recruitment. Not absolutely required for transcription in vitro, but particularly important in cases where Tbp or Tfb function is not optimal. It dynamically alters the nucleic acid-binding properties of RNA polymerases by stabilizing the initiation complex and destabilizing elongation complexes. Seems to translocate with the RNA polymerase following initiation and acts by binding to the non template strand of the transcription bubble in elongation complexes
Part of the Rad50 Mre11 complex, which is involved in the early steps of DNA double-strand break (DSB) repair. The complex may facilitate opening of the processed DNA ends to aid in the recruitment of HerA and NurA. Mre11 binds to DSB ends and has both double-stranded 3'-5' exonuclease activity and single- stranded endonuclease activity
Molecular chaperone capable of stabilizing a range of proteins. Seems to fulfill an ATP-independent, HSP70-like function in archaeal de novo protein folding
Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate- limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth
Involved in the biosynthesis of isoprenoids. Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its allylic isomer, dimethylallyl diphosphate (DMAPP)
Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules
Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double- stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA
Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP
Catalyzes the NAD(P)( )-dependent oxidation of D-glucose to D-gluconate via gluconolactone. Can utilize both NAD( ) and NADP( ) as electron acceptor. Is involved in the degradation of glucose through a modified Entner-Doudoroff pathway
A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner
K02469
-
5.99.1.3
4.871e-231
740.0
MMS1_k127_2692830_30
Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives
Catalyzes the deamination of 5-methylthioadenosine and S-adenosyl-L-homocysteine into 5-methylthioinosine and S-inosyl-L- homocysteine, respectively. Is also able to deaminate adenosine
Possesses two activities a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3' to 5' direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase
One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity
Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val)
Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by bleomycin and neocarzinostatin
NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient
NADP-dependent dehydrogenase of the nED (non- phosphorylated Entner-Doudoroff) pathway with highest activity towards glyceraldehydes (e.g. D,L-glyceraldehyde and D- glyceraldehyde), to a lesser extent towards D,L-glyceraldehyde-3- phosphate and glycolaldehyde, but no activity towards aliphatic or aromatic aldehydes
Sliding clamp subunit that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase and other proteins to DNA during high-speed replication
S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the trimethylation of the amino group of the modified target histidine residue in translation elongation factor 2 (EF- 2), to form an intermediate called diphthine. The three successive methylation reactions represent the second step of diphthamide biosynthesis
K20215
-
2.1.1.98
0.000000000000000000000000000000000007313
146.0
MMS1_k127_2692830_7
A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner
K02470
-
5.99.1.3
1.207e-212
678.0
MMS1_k127_2692830_70
Binds directly to 23S rRNA. Probably involved in E site tRNA release
Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors
K02864
-
-
0.00000000000000000000000000000000002536
145.0
MMS1_k127_2692830_72
-
-
-
-
0.00000000000000000000000000000000005123
141.0
MMS1_k127_2692830_73
DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function and is a proofreading 3'-5' exonuclease
K02342
-
2.7.7.7
0.0000000000000000000000000000000425
133.0
MMS1_k127_2692830_74
Belongs to the GHMP kinase family. Mevalonate kinase subfamily
TIGRFAM SUF system FeS assembly protein, NifU family
K04488
-
-
0.0000000000000000000000000000005625
126.0
MMS1_k127_2692830_77
Domain present in PSD-95, Dlg, and ZO-1/2.
-
-
-
0.000000000000000000000000000000771
140.0
MMS1_k127_2692830_78
D-aminoacyl-tRNA deacylase with broad substrate specificity. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo
K09716
-
3.1.1.96
0.000000000000000000000000000002809
128.0
MMS1_k127_2692830_79
Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA
Catalyzes the phosphorylation of (R)-mevalonate (MVA) to (R)-mevalonate 5-phosphate (MVAP). Functions in the mevalonate (MVA) pathway leading to isopentenyl diphosphate (IPP), a key precursor for the biosynthesis of isoprenoid compounds such as archaeal membrane lipids
ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins
Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate
Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates
Catalyzes the irreversible transfer of a propylamine group from the amino donor S-adenosylmethioninamine (decarboxy- AdoMet) to putrescine (1,4-diaminobutane) to yield spermidine
Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ
type I phosphodiesterase nucleotide pyrophosphatase
-
-
-
0.0000000000000000000000007243
120.0
MMS1_k127_446309_34
K transport systems
K10716
-
-
0.00000000000000000001788
96.0
MMS1_k127_446309_36
Ion channel
K10716
-
-
0.0000000000000000007366
96.0
MMS1_k127_446309_37
COG1522 Transcriptional regulators
K03718
-
-
0.00000000000000003622
87.0
MMS1_k127_446309_38
transposase
K07497
-
-
0.00000000000000116
79.0
MMS1_k127_446309_39
Polysaccharide biosynthesis protein
-
-
-
0.0000000000005736
82.0
MMS1_k127_446309_4
Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone
Broad-specificity nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates
K18532
-
2.7.4.3
0.000000000008705
73.0
MMS1_k127_446309_42
Protein of unknown function (DUF357)
K09728
-
-
0.0000000000284
66.0
MMS1_k127_446309_43
Type II secretion system (T2SS), protein F
K07333
-
-
0.00000000005293
74.0
MMS1_k127_446309_44
Type II secretion system
K07333
-
-
0.00000000021
72.0
MMS1_k127_446309_45
Ion channel
-
-
-
0.0000000003325
70.0
MMS1_k127_446309_46
Belongs to the phosphoglycerate mutase family
K15634
-
5.4.2.12
0.000000000595
68.0
MMS1_k127_446309_47
Bacterial regulatory protein, arsR family
-
-
-
0.00000001503
62.0
MMS1_k127_446309_48
eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA
Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis
Catalyzes the decarboxylation of S-adenosylmethionine to S-adenosylmethioninamine (dcAdoMet), the propylamine donor required for the synthesis of the polyamines spermine and spermidine from the diamine putrescine
K00797,K01611
-
2.5.1.16,4.1.1.50
0.000000000000000000000000001305
117.0
MMS1_k127_6998_14
Catalyzes the decarboxylation of S-adenosylmethionine to S-adenosylmethioninamine (dcAdoMet), the propylamine donor required for the synthesis of the polyamines spermine and spermidine from the diamine putrescine
K01611
-
4.1.1.50
0.00000000000000000000001385
103.0
MMS1_k127_6998_15
Domain in cystathionine beta-synthase and other proteins.
-
-
-
0.000000000791
66.0
MMS1_k127_6998_2
Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF- independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism
Catalyzes the reductive methylation of 2'-deoxyuridine- 5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis
Involved in the biosynthesis of branched-chain polyamines, which support the growth of thermophiles under high- temperature conditions. Catalyzes the sequential condensation of spermidine with the aminopropyl groups of decarboxylated S- adenosylmethionines to produce N(4)-bis(aminopropyl)spermidine via N(4)-aminopropylspermidine
K07057
-
2.5.1.128
0.00000000000000000000000000000000000000000001638
177.0
MMS1_k127_6998_9
TspO/MBR family
K05770
-
-
0.000000000000000000000000000000000000000001345
162.0
MMS1_k127_743597_0
AAA domain, putative AbiEii toxin, Type IV TA system
One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit
K02986
-
-
0.00000000000000000000000000000000000004231
149.0
MMS1_k127_743597_14
Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits
Nucleotidyl transferase AbiEii toxin, Type IV TA system
K09144
-
-
0.0000000000046
75.0
MMS1_k127_743597_18
ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins
K03686,K05516
-
-
0.0000000004601
74.0
MMS1_k127_743597_19
Transcriptional regulator
-
-
-
0.000005896
56.0
MMS1_k127_743597_2
Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit
Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit
amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile)
K01870
-
6.1.1.5
7.298e-213
698.0
MMS1_k127_786892_1
Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate
Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate
COG0537 Diadenosine tetraphosphate (Ap4A) hydrolase and other HIT family
K19710
-
2.7.7.53
0.0000000000000000000000001457
111.0
MMS1_k127_799438_0
Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity
Hydroxymethylglutaryl-coenzyme A synthase N terminal
K01641
-
2.3.3.10
0.00000006498
63.0
MMS1_k127_799438_12
Presenilin, signal peptide peptidase, family
-
-
-
0.000001072
61.0
MMS1_k127_799438_13
Promotes the exchange of GDP for GTP in EF-1-alpha GDP, thus allowing the regeneration of EF-1-alpha GTP that could then be used to form the ternary complex EF-1-alpha GTP AAtRNA
K03232
-
-
0.000002494
52.0
MMS1_k127_799438_14
MazG-like family
-
-
-
0.00002668
51.0
MMS1_k127_799438_15
ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner
Part of the Rad50 Mre11 complex, which is involved in the early steps of DNA double-strand break (DSB) repair. The complex may facilitate opening of the processed DNA ends to aid in the recruitment of HerA and NurA. Rad50 controls the balance between DNA end bridging and DNA resection via ATP-dependent structural rearrangements of the Rad50 Mre11 complex
K03546
-
-
0.00000000000000000000000000000001212
147.0
MMS1_k127_799438_7
Belongs to the universal ribosomal protein uL16 family
eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA
K03238
-
-
0.000000000000000000000000000002722
124.0
MMS1_k127_799438_9
Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits
K03236
-
-
0.0000000000000000000006205
98.0
MMS1_k127_87942_0
amino acids such as threonine, to avoid such errors, it has a posttransfer editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner
Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation
Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation
Belongs to the eukaryotic ribosomal protein eS24 family
K02974
-
-
0.0000000004444
65.0
MMS1_k127_949808_18
protease
K07052
-
-
0.000000001815
68.0
MMS1_k127_949808_19
Pfam:DUF552
K09152
-
-
0.00000002214
60.0
MMS1_k127_949808_2
Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)
Histone-like transcription factor (CBF/NF-Y) and archaeal histone
-
-
-
0.00000002423
58.0
MMS1_k127_949808_21
Belongs to the eukaryotic ribosomal protein eS31 family
K02977
-
-
0.0000009366
51.0
MMS1_k127_949808_22
guanyl-nucleotide exchange factor activity
K00504,K01181,K06889,K07004,K12548
-
1.14.17.3,3.2.1.8
0.00002596
57.0
MMS1_k127_949808_23
LamG domain protein jellyroll fold domain protein
-
-
-
0.00006407
57.0
MMS1_k127_949808_24
NUDIX domain
-
-
-
0.0002447
49.0
MMS1_k127_949808_25
COG3209 Rhs family protein
-
-
-
0.0004411
54.0
MMS1_k127_949808_3
Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity
Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity
Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline
Involved in pre-rRNA and tRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in rRNA and tRNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA
amino acids such as threonine, to avoid such errors, it has a posttransfer editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner
