This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits
Binds 23S rRNA and is also seen to make contacts with the A and possibly P site tRNAs
K02878
-
-
0.00000000000000000000000000000000000000000003278
164.0
SRR25158408_k127_1071452_12
Inositol monophosphatase family
K01082
-
3.1.3.7
0.000000000000000000000000000000000000000000757
168.0
SRR25158408_k127_1071452_13
Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate
Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase
K02356
-
-
0.000000000000000000000000000000002484
138.0
SRR25158408_k127_1071452_19
IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins
K02520
-
-
0.00000000000000000000000000000001471
132.0
SRR25158408_k127_1071452_2
Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage
Plays an important role in DNA replication, recombination and repair. Binds to ssDNA and to an array of partner proteins to recruit them to their sites of action during DNA metabolism
K03111
-
-
0.0000000000000000000000000000006507
126.0
SRR25158408_k127_1071452_23
One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit
K02994
-
-
0.00000000000000000000000000001374
122.0
SRR25158408_k127_1071452_24
This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance
One of the proteins that surrounds the polypeptide exit tunnel on the outside of the subunit
K02895
-
-
0.0000000000000000000000000004665
116.0
SRR25158408_k127_1071452_26
Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA
K02965
-
-
0.00000000000000000000000005109
110.0
SRR25158408_k127_1071452_27
The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome
K02890
-
-
0.000000000000000000000007041
104.0
SRR25158408_k127_1071452_28
Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit
K02887
-
-
0.00000000000000000000001263
104.0
SRR25158408_k127_1071452_29
Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism
The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently
This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7 L12 stalk, and near the tRNA binding site of the peptidyltransferase center
Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit
One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, probably blocks exit of the E-site tRNA
amino acids such as threonine, to avoid such errors, it has a posttransfer editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner
Belongs to the small heat shock protein (HSP20) family
K13993
-
-
0.0000000000000008049
83.0
SRR25158408_k127_1360431_2
Protease prsW family
-
-
-
0.00002615
54.0
SRR25158408_k127_1491839_0
Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane
Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln)
An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. Plays a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control
Attaches a formyl group to the free amino group of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus
Raf kinase inhibitor-like protein, YbhB YbcL family
K06910
-
-
0.0000000000000000000000000000000000002738
150.0
SRR25158408_k127_1493945_2
Involved in the restart of stalled replication forks. Recognizes and binds the arrested nascent DNA chain at stalled replication forks. It can open the DNA duplex, via its helicase activity, and promote assembly of the primosome and loading of the major replicative helicase DnaB onto DNA
K04066
-
-
0.000000000000000000000000009136
123.0
SRR25158408_k127_1493945_3
Removes the formyl group from the N-terminal Met of newly synthesized proteins
Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome
Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth
Required for disulfide bond formation in some periplasmic proteins. Acts by transferring its disulfide bond to other proteins and is reduced in the process
-
-
-
0.000000000000000000000000000000000000000000112
168.0
SRR25158408_k127_1840166_7
RNase_H superfamily
-
-
-
0.0000000000000000000000000000000000625
140.0
SRR25158408_k127_1840166_8
COG0530 Ca2 Na antiporter
K07301
-
-
0.0000000000000000000001657
108.0
SRR25158408_k127_1840166_9
Specifically methylates the 50S ribosomal protein L3 on a specific glutamine residue
K07320
-
2.1.1.298
0.0000000000004481
74.0
SRR25158408_k127_1897273_0
Domain of unknown function (DUF4215)
-
-
-
0.00000000000008249
83.0
SRR25158408_k127_1897273_1
calcium- and calmodulin-responsive adenylate cyclase activity
-
-
-
0.000000004634
69.0
SRR25158408_k127_1897273_2
Bacterial Ig-like domain (group 3)
K20276
-
-
0.00004162
56.0
SRR25158408_k127_1915340_0
Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions
An RNase that has 5'-3' exonuclease and possibly endonuclease activity. Involved in maturation of rRNA and in some organisms also mRNA maturation and or decay
Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family
K03218
-
2.1.1.185
0.00000000000000000000000000000000000000003545
161.0
SRR25158408_k127_1915340_15
HAD-superfamily subfamily IB hydrolase, TIGR01490
-
-
-
0.000000000000000000000000000000000000003981
155.0
SRR25158408_k127_1915340_16
Predicted 3'-5' exonuclease related to the exonuclease domain of PolB
K07501
-
-
0.0000000000000000000000000000000008175
141.0
SRR25158408_k127_1915340_18
Diguanylate cyclase
-
-
-
0.00000000000000000000000000000007868
132.0
SRR25158408_k127_1915340_19
-
-
-
-
0.0000000000000000000000000000004017
135.0
SRR25158408_k127_1915340_2
Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE
DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N- glycosidic bond, leaving an AP (apurinic apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing
RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme
Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)
Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria
Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group
K01159
-
3.1.22.4
0.00000000000000000000000000007695
121.0
SRR25158408_k127_2022376_9
Required for the insertion and or proper folding and or complex formation of integral membrane proteins into the membrane. Involved in integration of membrane proteins that insert both dependently and independently of the Sec translocase complex, as well as at least some lipoproteins. Aids folding of multispanning membrane proteins
K03217
-
-
0.00000000006993
72.0
SRR25158408_k127_2040571_0
Catalyzes the oxidation of glucose 6-phosphate to 6- phosphogluconolactone
EVIDENCE EXPERIMENTAL PMID 1328163 BIO14.04 Transposon related functions. BELONGS TO THE IS3 IS150 IS904 FAMILY OF TRANSPOSASE. There are 9 such elements in the chromosome
Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation
K01874
-
6.1.1.10
0.00000000000000000000001805
104.0
SRR25158408_k127_2040571_6
PFAM Glycosyl transferase, group 1
-
-
-
0.000000000000000000003507
100.0
SRR25158408_k127_2040571_7
Could be a nuclease involved in processing of the 5'-end of pre-16S rRNA
Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits
Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc- (pentapeptide)GlcNAc (lipid intermediate II)
DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function and is a proofreading 3'-5' exonuclease
This protein binds to 23S rRNA in the presence of protein L20
K02888
-
-
0.0000000000000000000005121
100.0
SRR25158408_k127_2451526_25
Participates in chromosomal partition during cell division. May act via the formation of a condensin-like complex containing Smc and ScpB that pull DNA away from mid-cell into both cell halves
K05896
-
-
0.000000000000000000001223
104.0
SRR25158408_k127_2451526_26
Binds to the 23S rRNA
K02939
-
-
0.00000000000000000001592
96.0
SRR25158408_k127_2451526_27
Belongs to the bacterial ribosomal protein bL27 family
Participates in chromosomal partition during cell division. May act via the formation of a condensin-like complex containing Smc and ScpA that pull DNA away from mid-cell into both cell halves
Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)
Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides
Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine
The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by uvrB, the uvrA molecules dissociate
K03701
-
-
5.226e-290
914.0
SRR25158408_k127_3243477_1
damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage
K03702
-
-
1.118e-224
715.0
SRR25158408_k127_3243477_10
AI-2E family transporter
-
-
-
0.000000000000000000000000000000000001893
151.0
SRR25158408_k127_3243477_11
Peptidyl-prolyl cis-trans isomerase
K01802,K03772
-
5.2.1.8
0.0000000000000000000000000000000005435
137.0
SRR25158408_k127_3243477_12
F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation
Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane
K20276
-
-
0.0000000000001026
86.0
SRR25158408_k127_3243477_15
F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation
K02113
-
-
0.000002907
54.0
SRR25158408_k127_3243477_16
Component of the F(0) channel, it forms part of the peripheral stalk, linking F(1) to F(0)
K02109
-
-
0.00001316
53.0
SRR25158408_k127_3243477_17
Pterin 4 alpha carbinolamine dehydratase
K01724
-
4.2.1.96
0.00002795
50.0
SRR25158408_k127_3243477_18
Bacterial protein of unknown function (DUF916)
-
-
-
0.00003681
56.0
SRR25158408_k127_3243477_19
calcium- and calmodulin-responsive adenylate cyclase activity
it plays a direct role in the translocation of protons across the membrane
K02108
-
-
0.0000000000000000000000000000000000000000003958
167.0
SRR25158408_k127_3243477_6
enzyme of poly-gamma-glutamate biosynthesis (capsule formation)
K07282
-
-
0.00000000000000000000000000000000000000001434
167.0
SRR25158408_k127_3243477_7
The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision
K03703
-
-
0.0000000000000000000000000000000000000122
160.0
SRR25158408_k127_3243477_8
cytochrome c biogenesis protein
K06196
-
-
0.0000000000000000000000000000000000002285
151.0
SRR25158408_k127_3243477_9
Histidine kinase
-
-
-
0.0000000000000000000000000000000000006896
155.0
SRR25158408_k127_327193_0
Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)
Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity
Single strand-specific metallo-endoribonuclease involved in late-stage 70S ribosome quality control and in maturation of the 3' terminus of the 16S rRNA
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction
Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates
ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins
One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit
K02986
-
-
0.0000000000000000000000000000000000004647
148.0
SRR25158408_k127_3872521_15
Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits
K02952
-
-
0.0000000000000000000000000000001708
127.0
SRR25158408_k127_3872521_16
Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex
Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine-Dalgarno cleft in the 70S ribosome
K02948
-
-
0.0000000000000000000000000000002093
127.0
SRR25158408_k127_3872521_18
Peptidase M16 inactive domain
-
-
-
0.000000000000000000000000001568
126.0
SRR25158408_k127_3872521_19
Ribosomal protein L17
K02879
-
-
0.0000000000000000000000002958
108.0
SRR25158408_k127_3872521_2
Belongs to the class-I aminoacyl-tRNA synthetase family
K01869
-
6.1.1.4
1.04e-218
703.0
SRR25158408_k127_3872521_20
Vitamin k epoxide reductase
-
-
-
0.000000000000000000000001799
108.0
SRR25158408_k127_3872521_21
Belongs to the universal ribosomal protein uS9 family
This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly
One of the essential components for the initiation of protein synthesis. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps modulate mRNA selection, yielding the 30S pre- initiation complex (PIC). Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are released leaving the mature 70S translation initation complex
Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another
Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides
K03624
-
-
0.000000000000000000000000000003564
127.0
SRR25158408_k127_4280385_19
MgtC family
K07507
-
-
0.000000000000000000000000004104
118.0
SRR25158408_k127_4280385_2
Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family
Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs
K03177
-
5.4.99.25
0.000000000000000002525
96.0
SRR25158408_k127_4280385_22
Lytic transglycosylase with a strong preference for naked glycan strands that lack stem peptides
K03642
-
-
0.000000000000001795
82.0
SRR25158408_k127_4280385_23
HxlR-like helix-turn-helix
-
-
-
0.0000000000002416
74.0
SRR25158408_k127_4280385_24
sequence-specific DNA binding
-
-
-
0.00000023
55.0
SRR25158408_k127_4280385_25
Type II restriction enzyme, methylase subunits
-
-
-
0.0000002377
54.0
SRR25158408_k127_4280385_26
Barrel-sandwich domain of CusB or HlyD membrane-fusion
K02005
-
-
0.0000002595
63.0
SRR25158408_k127_4280385_27
Involved in formation and maintenance of cell shape
K03570
-
-
0.000003473
58.0
SRR25158408_k127_4280385_28
there are paralogous genes in several bacterial genomes, and a CXXC motif for zinc binding and an upstream regulation region of the paralog lacking this motif that are regulated by zinc
Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE
K03695
-
-
3.769e-287
908.0
SRR25158408_k127_4300256_1
One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex
Master enzyme that delivers sulfur to a number of partners involved in Fe-S cluster assembly, tRNA modification or cofactor biosynthesis. Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Functions as a sulfur delivery protein for Fe-S cluster synthesis onto IscU, an Fe-S scaffold assembly protein, as well as other S acceptor proteins
Belongs to the protein kinase superfamily. Ser Thr protein kinase family
-
-
-
0.00000000001238
72.0
SRR25158408_k127_4300256_5
One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Required for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA
K02834
-
-
0.000001456
54.0
SRR25158408_k127_437511_0
DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA
Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln)
Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln)
K02435
-
6.3.5.6,6.3.5.7
0.0000000351
58.0
SRR25158408_k127_437511_4
peptidase
K02654
-
3.4.23.43
0.000107
49.0
SRR25158408_k127_4416805_0
A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner
Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone
In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance
Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec)
DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity
Critical role in recombination and DNA repair. Helps process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3'- to 5'- polarity. Unwinds branched duplex DNA (Y-DNA)
COG0265 Trypsin-like serine proteases, typically periplasmic, contain C-terminal PDZ domain
-
-
-
0.0002573
53.0
SRR25158408_k127_4773148_2
Magnesium transport protein CorA
K03284
-
-
0.0000000000000000000000000000000000000000005538
170.0
SRR25158408_k127_4773148_3
Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions
K03530
-
-
0.00000000000000000000000006967
109.0
SRR25158408_k127_4773148_4
Serine hydrolase
K07002
-
-
0.0000000000000000003281
97.0
SRR25158408_k127_4773148_5
Acetyltransferase (GNAT) domain
-
-
-
0.00000000000000002149
89.0
SRR25158408_k127_4773148_6
Belongs to the UPF0102 family
K07460
-
-
0.00000000004635
68.0
SRR25158408_k127_4773148_7
RNA polymerase sigma-70 factor, ECF subfamily
K03088
-
-
0.00000003819
63.0
SRR25158408_k127_4773148_8
Transposase
-
-
-
0.0001007
52.0
SRR25158408_k127_4773148_9
Cupin 2, conserved barrel domain protein
-
-
-
0.0001219
48.0
SRR25158408_k127_4820082_0
Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins
the 2 termini fold to resemble tRNA(Ala) and it encodes a tag peptide , a short internal open reading frame. During trans-translation Ala- aminoacylated tmRNA acts like a tRNA, entering the A-site of stalled ribosomes, displacing the stalled mRNA. The ribosome then switches to translate the ORF on the tmRNA
K03664
-
-
0.0000000000000000000000000000003831
128.0
SRR25158408_k127_4820082_3
response to heat
K03668,K03929
-
-
0.00000000000000000000004194
107.0
SRR25158408_k127_4820082_4
ig-like, plexins, transcription factors
-
-
-
0.0000000000000000000001034
106.0
SRR25158408_k127_4820082_5
N-acetylmuramoyl-L-alanine amidase
K01448
-
3.5.1.28
0.000000008682
65.0
SRR25158408_k127_4866698_0
Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome
K02355
-
-
2.435e-261
824.0
SRR25158408_k127_4866698_1
This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis
ABC-type transport system involved in lipoprotein release permease component
K02004
-
-
0.00000000000000000000000000408
125.0
SRR25158408_k127_4866698_6
Belongs to the NAD(P)-dependent epimerase dehydratase family
-
-
-
0.00000000000000000000000003718
119.0
SRR25158408_k127_4866698_7
Alternative oxidase
-
-
-
0.0000000000000000000007802
104.0
SRR25158408_k127_4866698_8
Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family
-
-
-
0.0000000000000008514
91.0
SRR25158408_k127_4866698_9
Transposase, Mutator family of Pasteurellaceae UniRef RepID F4H9B5_GALAU
-
-
-
0.000000002886
62.0
SRR25158408_k127_4887782_0
Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane
Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins
Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P)
Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation. Functions as a peptidyl-prolyl cis-trans isomerase
One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity
One of the early assembly proteins it binds 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome. Forms the main docking site for trigger factor binding to the ribosome
Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell
Essential subunit of the Sec protein translocation channel SecYEG. Clamps together the 2 halves of SecY. May contact the channel plug during translocation
Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis
K00287
-
1.5.1.3
0.0000000000000000000000000000000001424
140.0
SRR25158408_k127_807315_11
MazG nucleotide pyrophosphohydrolase domain
-
-
-
0.0000000000000000000000000000000001432
135.0
SRR25158408_k127_807315_12
Domain of unknown function DUF11
-
-
-
0.000009018
59.0
SRR25158408_k127_807315_13
Involved in DNA repair and RecF pathway recombination
K03584
-
-
0.0007529
49.0
SRR25158408_k127_807315_2
Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF- independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism
Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation
Catalyzes the reductive methylation of 2'-deoxyuridine- 5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis
Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis
K00943
-
2.7.4.9
0.00000000000000000000000000000000000000000009647
167.0
SRR25158408_k127_850554_0
Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner
Involved in peptidoglycan biosynthesis. Transports lipid-linked peptidoglycan precursors from the inner to the outer leaflet of the cytoplasmic membrane
Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA
Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate
K01491
-
1.5.1.5,3.5.4.9
0.00000000000000000000000000000000000000000008396
170.0
SRR25158408_k127_877266_7
Glycosyl transferases group 1
-
-
-
0.0000000000000000000000000000000000000000142
168.0
SRR25158408_k127_877266_8
Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Is thus essential for accurate translation
A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner
Belongs to the bacterial ribosomal protein bL32 family
K02911
GO:0003674,GO:0003735,GO:0005198
-
0.000000009261
61.0
SRR25158408_k127_909496_12
Sortase family
K07284
-
3.4.22.70
0.00000715
56.0
SRR25158408_k127_909496_13
Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane
K20276
-
-
0.000009697
58.0
SRR25158408_k127_909496_2
Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr)
Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre- crRNA and tracrRNA of type II CRISPR loci if present in the organism
Involved in transcription antitermination. Required for transcription of ribosomal RNA (rRNA) genes. Binds specifically to the boxA antiterminator sequence of the ribosomal RNA (rrn) operons
