Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
-
-
-
0.000000000000000008692
95.0
WLSH1_k127_10050630_1
Transcriptional regulatory protein, C terminal
-
-
-
0.0000000000004708
69.0
WLSH1_k127_10050630_2
Transcriptional regulatory protein, C terminal
K07664
-
-
0.000000000002927
68.0
WLSH1_k127_10050630_3
Transcriptional regulatory protein, C terminal
K07668,K07775
-
-
0.0000000003237
61.0
WLSH1_k127_10050630_4
phosphorelay signal transduction system
-
-
-
0.00001681
48.0
WLSH1_k127_1005873_0
Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and or for immediate growth after restoration of oxygen
K00525
-
1.17.4.1
0.0000000000000000000000000000000000002654
145.0
WLSH1_k127_10076859_0
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates
An essential GTPase that binds both GDP and GTP, with rapid nucleotide exchange. Plays a role in 16S rRNA processing and 30S ribosomal subunit biogenesis and possibly also in cell cycle regulation and energy metabolism
K03595
-
-
0.00000000000000002122
86.0
WLSH1_k127_10218627_1
DNA primase activity
K02316
-
-
0.0000000000000004256
80.0
WLSH1_k127_10218627_2
COG0500 SAM-dependent methyltransferases
-
-
-
0.00000000001231
74.0
WLSH1_k127_10218627_3
An essential GTPase that binds both GDP and GTP, with rapid nucleotide exchange. Plays a role in 16S rRNA processing and 30S ribosomal subunit biogenesis and possibly also in cell cycle regulation and energy metabolism
K03595
-
-
0.000000001022
61.0
WLSH1_k127_10218627_4
2 iron, 2 sulfur cluster binding
K13771
-
-
0.000001576
51.0
WLSH1_k127_10218627_5
RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication
K02316
-
-
0.00000698
50.0
WLSH1_k127_10218627_6
An essential GTPase that binds both GDP and GTP, with rapid nucleotide exchange. Plays a role in 16S rRNA processing and 30S ribosomal subunit biogenesis and possibly also in cell cycle regulation and energy metabolism
K03595
-
-
0.000392
44.0
WLSH1_k127_10289266_0
Belongs to the pyruvate kinase family
K00873
-
2.7.1.40
0.000000000000000000000000000000000000001545
153.0
WLSH1_k127_10322098_0
The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently
This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7 L12 stalk, and near the tRNA binding site of the peptidyltransferase center
Located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body
K02988
-
-
0.000000000000000000000000000000000003804
146.0
WLSH1_k127_10322098_3
This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance
ATPase, P-type (transporting), HAD superfamily, subfamily IC
K17686
-
3.6.3.54
0.00000000000000000000000000000000000000158
150.0
WLSH1_k127_10393863_3
PFAM alkyl hydroperoxide reductase Thiol specific antioxidant Mal allergen
K03564
-
1.11.1.15
0.0000000000000000818
81.0
WLSH1_k127_10393863_4
bacterioferritin comigratory protein
K03564
-
1.11.1.15
0.000000003767
59.0
WLSH1_k127_10393863_5
Heavy-metal-associated domain
K17686
-
3.6.3.54
0.000000007438
57.0
WLSH1_k127_10393863_6
copper-exporting ATPase
K01533,K17686
-
3.6.3.4,3.6.3.54
0.0003061
43.0
WLSH1_k127_10453458_0
Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain
Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group
K01159
-
3.1.22.4
0.000000000000000001403
89.0
WLSH1_k127_10453458_2
Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group
catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A- site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome
Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome
Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism
K00939
-
2.7.4.3
0.0000000000000000000002925
103.0
WLSH1_k127_10741888_1
HemN C-terminal domain
-
-
-
0.000000000000000001257
90.0
WLSH1_k127_10741888_2
HemN C-terminal domain
-
-
-
0.00000000000000004855
86.0
WLSH1_k127_10741888_3
Involved in the biosynthesis of porphyrin-containing compound
The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing
Required for disulfide bond formation in some periplasmic proteins. Acts by transferring its disulfide bond to other proteins and is reduced in the process
Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate
Provides the (R)-glutamate required for cell wall biosynthesis
K01776
-
5.1.1.3
0.0000000000000699
71.0
WLSH1_k127_10997949_0
A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner
Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates
amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile)
COGs COG1253 Hemolysins and related protein containing CBS domains
-
-
-
0.000000000000000000000000009498
116.0
WLSH1_k127_1224605_1
Heat shock 70 kDa protein
K04043
-
-
0.000000000000000367
78.0
WLSH1_k127_1247476_0
protein serine/threonine kinase activity
-
-
-
0.00007774
45.0
WLSH1_k127_1256075_0
Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage
Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage
Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S- adenosylhomocysteine (SAH AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively
K01243
-
3.2.2.9
0.00000003487
59.0
WLSH1_k127_1437362_2
Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S- adenosylhomocysteine (SAH AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively
K01243
-
3.2.2.9
0.0006968
44.0
WLSH1_k127_1446590_0
Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)
K00791
-
2.5.1.75
0.00000000000000006098
83.0
WLSH1_k127_1446590_1
Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)
K00791
-
2.5.1.75
0.00000000000000006875
82.0
WLSH1_k127_1446590_2
Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)
Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)
K00791
-
2.5.1.75
0.00000000002664
64.0
WLSH1_k127_1446590_4
Polysaccharide pyruvyl transferase
-
-
-
0.00001025
51.0
WLSH1_k127_1465986_0
First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan
Flavin transferase that catalyzes the transfer of the FMN moiety of FAD and its covalent binding to the hydroxyl group of a threonine residue in a target flavoprotein
K03734
-
2.7.1.180
0.0000000000000000000000000001531
121.0
WLSH1_k127_1472378_1
Flavin transferase that catalyzes the transfer of the FMN moiety of FAD and its covalent binding to the hydroxyl group of a threonine residue in a target flavoprotein
K03734
-
2.7.1.180
0.000000006735
63.0
WLSH1_k127_1472378_2
FMN_bind
-
-
-
0.000402
53.0
WLSH1_k127_1493314_0
Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)
K01925
-
6.3.2.9
0.00000000000004773
76.0
WLSH1_k127_1493314_1
Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)
K01925
-
6.3.2.9
0.0000000007761
65.0
WLSH1_k127_1493314_2
Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)
K01925
-
6.3.2.9
0.000007015
52.0
WLSH1_k127_1535378_0
Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)
Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)
Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit
K02887
-
-
0.0000000000000000000000000000785
120.0
WLSH1_k127_1716676_2
Could be involved in insertion of integral membrane proteins into the membrane
amino acids such as threonine, to avoid such errors, it has a posttransfer editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner
DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N- glycosidic bond, leaving an AP (apurinic apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
K10773
-
4.2.99.18
0.0000000000000000000000000164
113.0
WLSH1_k127_1829863_2
DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N- glycosidic bond, leaving an AP (apurinic apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
K10773
-
4.2.99.18
0.00000000000000000000002184
102.0
WLSH1_k127_1829863_3
TPM domain
K06872
-
-
0.0000000000009285
72.0
WLSH1_k127_1829863_4
LemA family
K03744
-
-
0.00000000000108
71.0
WLSH1_k127_1829863_5
LemA family
K03744
-
-
0.000000002332
61.0
WLSH1_k127_1832878_0
Metal-sensitive transcriptional repressor
K21600
-
-
0.000000000001156
71.0
WLSH1_k127_1832878_1
Short C-terminal domain
K08982
-
-
0.00000002536
60.0
WLSH1_k127_1832878_2
ABC-type oligopeptide transport system, periplasmic component
In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance
Functions as a peptidoglycan terminase that cleaves nascent peptidoglycan strands endolytically to terminate their elongation
K07082
-
-
0.0000000000000000000000000000000000002555
150.0
WLSH1_k127_2181646_0
Catalyzes the ATP- as well as the pyrophosphate- dependent phosphorylation of a specific serine residue in HPr, a phosphocarrier protein of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS). HprK P also catalyzes the pyrophosphate-producing, inorganic phosphate-dependent dephosphorylation (phosphorolysis) of seryl-phosphorylated HPr (P- Ser-HPr). The two antagonistic activities of HprK P are regulated by several intracellular metabolites, which change their concentration in response to the absence or presence of rapidly metabolisable carbon sources (glucose, fructose, etc.) in the growth medium. Therefore, by controlling the phosphorylation state of HPr, HPrK P is a sensor enzyme that plays a major role in the regulation of carbon metabolism and sugar transport it mediates carbon catabolite repression (CCR), and regulates PTS-catalyzed carbohydrate uptake and inducer exclusion
Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys- tRNA(Pro) is not edited by ProRS
PFAM pyruvate flavodoxin ferredoxin oxidoreductase domain protein
K00174
-
1.2.7.11,1.2.7.3
0.0003888
47.0
WLSH1_k127_2528068_0
transcriptional regulator
-
-
-
0.00000000000000000000000000000000000001026
149.0
WLSH1_k127_2562403_0
Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA
Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA
Transfers the N-acyl diglyceride group on what will become the N-terminal cysteine of membrane lipoproteins
K13292
-
-
0.0003029
50.0
WLSH1_k127_2587639_0
PFAM alkyl hydroperoxide reductase Thiol specific antioxidant Mal allergen
K03386
-
1.11.1.15
0.000000000000000000000000000000000000000509
152.0
WLSH1_k127_2608710_0
DNA polymerase III alpha subunit
K02337,K14162
-
2.7.7.7
0.0001814
51.0
WLSH1_k127_2617832_0
TIGRFAM stage V sporulation protein D
K03587,K08384
-
3.4.16.4
0.0000000000000000000000001621
109.0
WLSH1_k127_2617832_1
PFAM penicillin-binding protein transpeptidase, Penicillin-binding protein dimerization domain-containing protein, PASTA domain containing protein
K08384
-
-
0.0000000000000000000004217
105.0
WLSH1_k127_2617832_2
Penicillin-binding Protein dimerisation domain
K03587
-
3.4.16.4
0.0000000000001414
73.0
WLSH1_k127_2617832_3
Penicillin-binding protein, transpeptidase domain protein
K03587
-
3.4.16.4
0.0000000008966
61.0
WLSH1_k127_2620214_0
In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance
Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine
Pfam Adenylate and Guanylate cyclase catalytic domain
K01768
-
4.6.1.1
0.00000000000000000189
89.0
WLSH1_k127_2684399_2
CHASE2
K01768
-
4.6.1.1
0.00000000000000007842
80.0
WLSH1_k127_2689059_0
Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction
K00962
-
2.7.7.8
0.0000000000000000000000000000502
120.0
WLSH1_k127_2689059_1
Putative RNA methylase family UPF0020
-
-
-
0.0009364
50.0
WLSH1_k127_2701211_0
Required for the insertion and or proper folding and or complex formation of integral membrane proteins into the membrane. Involved in integration of membrane proteins that insert both dependently and independently of the Sec translocase complex, as well as at least some lipoproteins. Aids folding of multispanning membrane proteins
K03217
-
-
0.00000000000000002695
93.0
WLSH1_k127_273260_0
Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth
RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication
K02316
-
-
0.0000000000000000000003872
97.0
WLSH1_k127_273260_3
RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication
K02316
-
-
0.00002074
47.0
WLSH1_k127_2771904_0
Provides the (R)-glutamate required for cell wall biosynthesis
K01776
-
5.1.1.3
0.0000000001271
64.0
WLSH1_k127_2771904_1
Provides the (R)-glutamate required for cell wall biosynthesis
K01776
-
5.1.1.3
0.0000004489
52.0
WLSH1_k127_2778189_0
repeat protein
-
-
-
0.00006142
47.0
WLSH1_k127_2822282_0
Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins
Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit
One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, probably blocks exit of the E-site tRNA
K02992
-
-
0.00000000000000000000000000000000002391
139.0
WLSH1_k127_2945653_2
Belongs to the bacterial ribosomal protein bL27 family
Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria
Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria
Domain present in phytochromes and cGMP-specific phosphodiesterases.
-
-
-
0.000000004258
59.0
WLSH1_k127_3221856_1
Diguanylate cyclase phosphodiesterase with PAS PAC
-
-
-
0.0005305
48.0
WLSH1_k127_3226677_0
Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE
transferase activity, transferring glycosyl groups
K12994
-
2.4.1.349
0.000003363
50.0
WLSH1_k127_3272782_0
An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. Plays a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control
Couples transcription and DNA repair by recognizing RNA polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent release of RNAP and its truncated transcript from the DNA, and recruitment of nucleotide excision repair machinery to the damaged site
K03723
-
-
0.000000000000000000000000000689
121.0
WLSH1_k127_3490146_2
Psort location Cytoplasmic, score
K00588
-
2.1.1.104
0.00000000000000000000003065
106.0
WLSH1_k127_3490146_3
Couples transcription and DNA repair by recognizing RNA polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent release of RNAP and its truncated transcript from the DNA, and recruitment of nucleotide excision repair machinery to the damaged site
K03723
-
-
0.00000000001081
65.0
WLSH1_k127_3490146_4
Produces ATP from ADP in the presence of a proton gradient across the membrane
Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone
Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides
K01255
-
3.4.11.1
0.00000000000000000000000001813
111.0
WLSH1_k127_3610327_1
Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides
Catalyzes the condensation of isopentenyl diphosphate (IPP) with allylic pyrophosphates generating different type of terpenoids
K00806
-
2.5.1.31
0.000000000000000000000000000000003556
133.0
WLSH1_k127_3751192_1
Participates in both transcription termination and antitermination
K02600
-
-
0.000000000000000000000000000000004959
132.0
WLSH1_k127_3751192_2
the 2 termini fold to resemble tRNA(Ala) and it encodes a tag peptide , a short internal open reading frame. During trans-translation Ala- aminoacylated tmRNA acts like a tRNA, entering the A-site of stalled ribosomes, displacing the stalled mRNA. The ribosome then switches to translate the ORF on the tmRNA
K03664
-
-
0.0000000000000000000000000003526
117.0
WLSH1_k127_3751192_3
domain protein
K06881
-
3.1.13.3,3.1.3.7
0.0000000000000000001163
94.0
WLSH1_k127_3751192_4
Likely ribonuclease with RNase H fold.
K07447
-
-
0.0000000000000001524
84.0
WLSH1_k127_3751192_5
Ribosomal protein L9, N-terminal domain
K02939
-
-
0.00000000229
65.0
WLSH1_k127_3751192_6
Catalyzes the condensation of isopentenyl diphosphate (IPP) with allylic pyrophosphates generating different type of terpenoids
K00806
-
2.5.1.31
0.000000003523
57.0
WLSH1_k127_3751192_7
DHH family
K06881
-
3.1.13.3,3.1.3.7
0.0002926
46.0
WLSH1_k127_3765678_0
Transcriptional regulatory protein, C terminal
-
-
-
0.000000000000000000003135
95.0
WLSH1_k127_3765678_1
response regulator, receiver
-
-
-
0.00000000000000002839
82.0
WLSH1_k127_3765678_2
Histidine kinase
-
-
-
0.00000000000002056
76.0
WLSH1_k127_3765678_3
protein histidine kinase activity
-
-
-
0.0000000001598
69.0
WLSH1_k127_3765678_4
May be involved in the formation or repair of Fe-S clusters present in iron-sulfur proteins
damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage
Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp Asn)
Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism
Involved in unsaturated fatty acids biosynthesis. Catalyzes the dehydration of short chain beta-hydroxyacyl-ACPs and long chain saturated and unsaturated beta-hydroxyacyl-ACPs
Involved in molybdopterin and thiamine biosynthesis, family 2
K21029
-
2.7.7.80
0.0000000000000000000000000000002136
134.0
WLSH1_k127_4251294_1
-
-
-
-
0.0000002374
58.0
WLSH1_k127_427105_0
An essential GTPase that binds both GDP and GTP, with rapid nucleotide exchange. Plays a role in 16S rRNA processing and 30S ribosomal subunit biogenesis and possibly also in cell cycle regulation and energy metabolism
A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner
K02469
-
5.99.1.3
2.746e-244
780.0
WLSH1_k127_4277004_1
cell division
-
-
-
0.0000000000000001424
90.0
WLSH1_k127_4277004_2
O-linked N-acetylglucosamine transferase SPINDLY family
-
-
-
0.0000001584
60.0
WLSH1_k127_429474_0
Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE
Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE
Peptidoglycan polymerase that is essential for cell division
K03588
-
-
0.0000000000000000004063
94.0
WLSH1_k127_429474_4
Belongs to the SEDS family
K03588
-
-
0.0000000000000001803
81.0
WLSH1_k127_429474_5
YGGT family
K02221
-
-
0.00003883
50.0
WLSH1_k127_4369546_0
ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins
Involved in formation and maintenance of cell shape
K03570
-
-
0.000001113
59.0
WLSH1_k127_4453291_0
Protein of unknown function (DUF4012)
-
-
-
0.000000000000006234
85.0
WLSH1_k127_4456273_0
Attaches a formyl group to the free amino group of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus
K00604
-
2.1.2.9
0.00000000000000000000000000000000000003887
146.0
WLSH1_k127_4456273_1
AI-2E family transporter
-
-
-
0.0000000000000001934
84.0
WLSH1_k127_4456273_2
Attaches a formyl group to the free amino group of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus
K00604
-
2.1.2.9
0.000000000002419
68.0
WLSH1_k127_4456273_3
Putative metal-binding motif
-
-
-
0.000000006253
60.0
WLSH1_k127_4488668_0
This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits
One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit
The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB
K03550
-
3.6.4.12
0.000000000000000000000000002512
118.0
WLSH1_k127_4823642_1
Polysaccharide pyruvyl transferase
-
-
-
0.00000002568
61.0
WLSH1_k127_4872659_0
Serine threonine protein kinase
K12132
-
2.7.11.1
0.0000000000000000000000000000000000000000004727
167.0
WLSH1_k127_4872659_1
Alginate export
-
-
-
0.000000000000000000000000000000005361
135.0
WLSH1_k127_4897199_0
DEAD-box RNA helicase possibly involved in RNA degradation. Unwinds dsRNA in both 5'- and 3'-directions, has RNA- dependent ATPase activity
Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate
Couples transcription and DNA repair by recognizing RNA polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent release of RNAP and its truncated transcript from the DNA, and recruitment of nucleotide excision repair machinery to the damaged site
K03723
-
-
0.00000000000000000000000000000002469
129.0
WLSH1_k127_4923367_4
Thioredoxin
K03671
-
-
0.0000000000000000000000000000007505
125.0
WLSH1_k127_4923367_5
Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate
K01491
-
1.5.1.5,3.5.4.9
0.0000000000006473
69.0
WLSH1_k127_4923367_6
Couples transcription and DNA repair by recognizing RNA polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent release of RNAP and its truncated transcript from the DNA, and recruitment of nucleotide excision repair machinery to the damaged site
K03723
-
-
0.0005513
51.0
WLSH1_k127_4937256_0
transferase activity, transferring glycosyl groups
-
-
-
0.00000000000000000000000000000000000000000136
174.0
WLSH1_k127_4937256_1
GTPase that associates with the 50S ribosomal subunit and may have a role during protein synthesis or ribosome biogenesis
Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln)
Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln)
Critical role in recombination and DNA repair. Helps process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3'- to 5'- polarity. Unwinds branched duplex DNA (Y-DNA)
Critical role in recombination and DNA repair. Helps process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3'- to 5'- polarity. Unwinds branched duplex DNA (Y-DNA)
Critical role in recombination and DNA repair. Helps process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3'- to 5'- polarity. Unwinds branched duplex DNA (Y-DNA)
K03655
-
3.6.4.12
0.00000000006096
69.0
WLSH1_k127_5337848_0
Tex-like protein N-terminal domain
K06959
-
-
0.00000000000000000000000000000001198
134.0
WLSH1_k127_5351769_0
amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile)
K01870
-
6.1.1.5
0.00000000000001526
77.0
WLSH1_k127_5351769_1
amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile)
K01870
-
6.1.1.5
0.0003881
44.0
WLSH1_k127_5351769_2
Phage minor structural protein
-
-
-
0.0005324
49.0
WLSH1_k127_5354643_0
peptidyl-tyrosine sulfation
-
-
-
0.0000000000000000001111
96.0
WLSH1_k127_5406392_0
ATP-dependent DNA helicase (RecQ)
K03654
-
3.6.4.12
0.0002677
44.0
WLSH1_k127_5420743_0
Catalyzes the conversion of cyclic dehypoxanthine futalosine (cyclic DHFL) into 1,4-dihydroxy-6-naphthoate, a step in the biosynthesis of menaquinone (MK, vitamin K2)
Participates in transcription elongation, termination and antitermination
K02601
-
-
0.000000000000000000000000000000000000000001609
162.0
WLSH1_k127_5548897_0
Belongs to the helicase family. UvrD subfamily
K03657
-
3.6.4.12
0.0000000000000000000003485
100.0
WLSH1_k127_5632662_0
Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates
Catalyzes hydrolysis of the D-alanyl-D-alanine dipeptide
K08641
-
3.4.13.22
0.00000000000000000000000000000001279
132.0
WLSH1_k127_5632662_4
Catalyzes hydrolysis of the D-alanyl-D-alanine dipeptide
K08641
-
3.4.13.22
0.00000000000004926
74.0
WLSH1_k127_5632662_5
Belongs to the LOG family
K06966
-
3.2.2.10
0.00001781
51.0
WLSH1_k127_5650778_0
Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation
Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome
This protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site
An RNase that has 5'-3' exonuclease and possibly endonuclease activity. Involved in maturation of rRNA and in some organisms also mRNA maturation and or decay
DNA repair enzyme involved in the repair of deaminated bases. Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA (By similarity)
Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol
-
-
-
0.00000000002571
67.0
WLSH1_k127_5779450_0
aminopeptidase activity
-
-
-
0.000000004349
67.0
WLSH1_k127_5779450_1
NLP P60 protein
-
-
-
0.0004867
52.0
WLSH1_k127_5779450_2
domain protein
-
-
-
0.0006445
51.0
WLSH1_k127_5856266_0
PFAM HI0933-like protein
K07007
-
-
0.000000000000000000000000000000000004229
148.0
WLSH1_k127_5856266_1
2-oxobutyrate metabolic process
K01733
-
4.2.3.1
0.0000000000000000000002344
101.0
WLSH1_k127_5856266_2
Putative diguanylate phosphodiesterase
-
-
-
0.0000000000000003825
88.0
WLSH1_k127_5856266_3
glycosylase
K03575
-
-
0.000000000000001517
79.0
WLSH1_k127_5856266_4
Flavoprotein family
K07007
-
-
0.000000000000009713
76.0
WLSH1_k127_5856266_5
base-excision repair
K03575
-
-
0.00000000001411
69.0
WLSH1_k127_5860253_0
Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln)
Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12
K14441
-
2.8.4.4
0.000000000000000000000000000000000000000287
159.0
WLSH1_k127_5873834_1
Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12
K14441
-
2.8.4.4
0.0000000000000000000000000000000003262
139.0
WLSH1_k127_5873834_2
FAD dependent oxidoreductase
K07137
-
-
0.00000000000000000000000001809
109.0
WLSH1_k127_5873834_3
Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln)
K02434
-
6.3.5.6,6.3.5.7
0.00000000000000000000000002438
112.0
WLSH1_k127_5873834_4
'oxidoreductase
K07137
-
-
0.000000000000000002551
85.0
WLSH1_k127_5900953_0
Periplasmic binding protein
K02016
-
-
0.000000000000000000000000000000000000000001173
158.0
WLSH1_k127_5900953_1
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction
K01409
-
2.3.1.234
0.00000000000000000000000001189
113.0
WLSH1_k127_5900953_2
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction
K01409
-
2.3.1.234
0.0001485
45.0
WLSH1_k127_5905163_0
multi-organism process
-
-
-
0.00000000000000000000000000000000000009559
147.0
WLSH1_k127_5905163_1
S4 domain
K14761
-
-
0.000000001814
62.0
WLSH1_k127_5908502_0
Subtilase family
-
-
-
0.00000006682
60.0
WLSH1_k127_5908502_1
domain protein
-
-
-
0.00001059
56.0
WLSH1_k127_5908502_2
Hint domain
-
-
-
0.00008125
51.0
WLSH1_k127_5915606_0
4Fe-4S single cluster domain of Ferredoxin I
-
-
-
0.00003916
48.0
WLSH1_k127_5915606_1
-
-
-
-
0.0002709
52.0
WLSH1_k127_5947915_0
regulation of ryanodine-sensitive calcium-release channel activity
-
-
-
0.0000005678
57.0
WLSH1_k127_5954809_0
Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins
Participates in both transcription termination and antitermination
K02600
-
-
0.0000000000000001594
84.0
WLSH1_k127_599178_0
DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA
K01972
-
6.5.1.2
0.000000000000000000000000000000002672
132.0
WLSH1_k127_599178_1
DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA
K01972
-
6.5.1.2
0.000000000000000000001394
96.0
WLSH1_k127_599178_2
DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA
DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA
K01972
-
6.5.1.2
0.0000000002756
63.0
WLSH1_k127_599178_4
DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA
DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA
K01972
-
6.5.1.2
0.0000005201
52.0
WLSH1_k127_599178_6
Prokaryotic N-terminal methylation motif
K02456,K02650,K02655
-
-
0.0003498
50.0
WLSH1_k127_5996232_0
COG0659 Sulfate permease and related
K03321
-
-
0.00000000000003001
83.0
WLSH1_k127_5996232_1
The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing
K03551
-
3.6.4.12
0.0000000000003798
70.0
WLSH1_k127_5996232_2
Sulfate permease family
-
-
-
0.0000002353
59.0
WLSH1_k127_6002811_0
Type II secretory pathway, ATPase PulE Tfp pilus assembly pathway, ATPase PilB
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides
K03768
-
5.2.1.8
0.00000000000000000000000000002156
117.0
WLSH1_k127_6064479_2
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides
K03768
-
5.2.1.8
0.000000000000000000004151
94.0
WLSH1_k127_6113885_0
von Willebrand factor type A domain
K07114
-
-
0.000000000004099
75.0
WLSH1_k127_6133201_0
COG1199 Rad3-related DNA helicases
-
-
-
0.0000000001131
74.0
WLSH1_k127_6156300_0
A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner
Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan
Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine
K03648
-
3.2.2.27
0.00000000000000000000000000000002191
126.0
WLSH1_k127_6215789_3
Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine
K03648
-
3.2.2.27
0.000000000000000000000000001786
111.0
WLSH1_k127_6215789_4
Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions
Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan
K01928
-
6.3.2.13
0.0000000000000000000002196
99.0
WLSH1_k127_6215789_6
Belongs to the class-II aminoacyl-tRNA synthetase family
K04567
-
6.1.1.6
0.0000000000000001019
85.0
WLSH1_k127_6215789_7
Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine
Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine
This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly
K02871
-
-
0.0000000000000000000000000000000000000003196
153.0
WLSH1_k127_6396715_2
ribosomal protein l17
K02879
-
-
0.0000000000000001724
83.0
WLSH1_k127_6396715_3
Belongs to the universal ribosomal protein uS9 family
K02996
-
-
0.000005384
49.0
WLSH1_k127_6404405_0
Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme
Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines
This protein binds to 23S rRNA in the presence of protein L20
K02888
-
-
0.00000000000000000006474
93.0
WLSH1_k127_6465418_2
Peptidase, M23
-
-
-
0.0000000000004611
78.0
WLSH1_k127_6465418_3
Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines
Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys- tRNA(Pro) is not edited by ProRS
Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)
Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)
K01881
-
6.1.1.15
0.00000000002167
68.0
WLSH1_k127_6551118_4
Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)
Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate
The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by UvrB, the UvrA molecules dissociate
The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by UvrB, the UvrA molecules dissociate
The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by UvrB, the UvrA molecules dissociate
ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins
Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane
Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane
Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving both as a receptor for the preprotein-SecB complex and as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane
Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34
K00566
-
2.8.1.13
0.0000000001483
63.0
WLSH1_k127_6953054_5
5' nucleotidase, deoxy (Pyrimidine), cytosolic type C protein (NT5C)
-
-
-
0.00000008343
59.0
WLSH1_k127_6953054_6
PFAM Peptidase family M23
-
-
-
0.000002015
55.0
WLSH1_k127_6956109_0
Glycoside-hydrolase family GH114
K21006
-
-
0.000000000005197
76.0
WLSH1_k127_6957421_0
NHL repeat
-
-
-
0.0000000000001
79.0
WLSH1_k127_6998509_0
PFAM F5 8 type C domain
-
-
-
0.000004933
49.0
WLSH1_k127_7008791_0
DDE superfamily endonuclease
-
-
-
0.000000005123
65.0
WLSH1_k127_7021425_0
ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins
ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins
K03686
-
-
0.0003276
44.0
WLSH1_k127_7030334_0
S-layer homology domain
-
-
-
0.0000000000000005776
90.0
WLSH1_k127_7030334_1
membrane
-
-
-
0.00000000000001827
85.0
WLSH1_k127_7037338_0
Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc- (pentapeptide)GlcNAc (lipid intermediate II)
K02563
-
2.4.1.227
0.000000000000000000000000000000004605
137.0
WLSH1_k127_7037338_1
Pfam:N_methyl_2
K10924
-
-
0.000000000000000000000000000000009319
131.0
WLSH1_k127_7037338_2
Single strand-specific metallo-endoribonuclease involved in late-stage 70S ribosome quality control and in maturation of the 3' terminus of the 16S rRNA
K01489,K07042
-
3.5.4.5
0.0000000000000000000004407
101.0
WLSH1_k127_7037338_3
Major Facilitator Superfamily
-
-
-
0.00000000000003743
79.0
WLSH1_k127_7037338_4
-
-
-
-
0.0000000000001539
71.0
WLSH1_k127_7037338_5
Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc- (pentapeptide)GlcNAc (lipid intermediate II)
K02563
-
2.4.1.227
0.000000004605
60.0
WLSH1_k127_7037338_6
-
-
-
-
0.00002409
48.0
WLSH1_k127_7063764_0
Polysaccharide biosynthesis protein
-
-
-
0.00000000668
68.0
WLSH1_k127_7063764_1
Polysaccharide biosynthesis protein
-
-
-
0.00007211
49.0
WLSH1_k127_7081881_0
Hydrolase, NUDIX family
K03574,K17816
-
3.6.1.55,3.6.1.56
0.000000000000000000000000000000000006783
141.0
WLSH1_k127_7081881_1
ABC transporter
-
-
-
0.00000000000000000000000000000002243
133.0
WLSH1_k127_7081881_2
ABC transporter, ATP-binding protein
-
-
-
0.0000000000000000000000000007335
119.0
WLSH1_k127_7081881_3
ABC transporter, ATP-binding protein
K06158
-
-
0.000000000000000001357
88.0
WLSH1_k127_7081881_4
ABC transporter C-terminal domain
K06158
-
-
0.00000003135
55.0
WLSH1_k127_7143065_0
CHASE2
K01768
-
4.6.1.1
0.00000001271
63.0
WLSH1_k127_7143065_1
CHASE2
K01768
-
4.6.1.1
0.000006032
54.0
WLSH1_k127_7175848_0
-
-
-
-
0.00000000000006046
84.0
WLSH1_k127_7209211_0
Cadherin domain
-
-
-
0.000000000000000000000000000006746
124.0
WLSH1_k127_7209211_1
ComF family
K02242
-
-
0.0000000001461
66.0
WLSH1_k127_7209211_2
competence protein
-
-
-
0.000316
44.0
WLSH1_k127_7211126_0
Large extracellular alpha-helical protein
-
-
-
0.000001985
59.0
WLSH1_k127_7211126_1
SPTR CHU large protein
-
-
-
0.000539
43.0
WLSH1_k127_7215386_0
Associated with various cellular activities
K03924
-
-
0.0000000000000000000000000000000000001338
142.0
WLSH1_k127_7215386_1
NmrA family
K19267
-
1.6.5.2
0.0000000000000000000000000000000001367
136.0
WLSH1_k127_7215386_2
NmrA-like family
K19267
-
1.6.5.2
0.00000000000000000002765
93.0
WLSH1_k127_7215386_3
ATPase family associated with various cellular activities (AAA)
K03924
-
-
0.00000000000000112
78.0
WLSH1_k127_7215386_4
NmrA-like family
K19267
-
1.6.5.2
0.0000000005415
61.0
WLSH1_k127_7215386_5
ATPase family associated with various cellular activities (AAA)
K03924
-
-
0.00000002746
56.0
WLSH1_k127_7244287_0
Transcriptional regulatory protein, C terminal
-
-
-
0.000000000000000000000000001589
119.0
WLSH1_k127_7244287_1
Transcriptional regulatory protein, C terminal
-
-
-
0.00000000000000000002919
98.0
WLSH1_k127_7244287_2
Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
-
-
-
0.0000004838
58.0
WLSH1_k127_7244287_3
His Kinase A (phosphoacceptor) domain
-
-
-
0.000001602
51.0
WLSH1_k127_7267043_0
A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner
A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner
Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P)
One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity
One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit
K02906
-
-
0.000000000000000000000000000000004528
134.0
WLSH1_k127_7369350_3
domain protein
-
-
-
0.00000000002823
68.0
WLSH1_k127_7369350_4
One of the early assembly proteins it binds 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome. Forms the main docking site for trigger factor binding to the ribosome
K02892
-
-
0.00000000008574
66.0
WLSH1_k127_7369350_5
One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity
Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp Asn)
Required for morphogenesis under gluconeogenic growth conditions
-
-
-
0.000000000001504
68.0
WLSH1_k127_7572230_0
Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D- arabino-heptulosonate-7-phosphate (DAHP)
transferase activity, transferring acyl groups other than amino-acyl groups
-
-
-
0.000000000000000000000000000000000001591
150.0
WLSH1_k127_7572230_2
Protein of unknown function DUF45
K07043
-
-
0.00000000000000000000000000000001307
130.0
WLSH1_k127_7572230_3
PFAM ABC transporter
K01990
-
-
0.000000000000000000002948
101.0
WLSH1_k127_7572230_4
Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D- arabino-heptulosonate-7-phosphate (DAHP)
Belongs to the UDP-glucose GDP-mannose dehydrogenase family
K00012
-
1.1.1.22
0.00000000000000000000000000001822
119.0
WLSH1_k127_7730952_0
tail specific protease
K03797
-
3.4.21.102
0.0000000000001221
78.0
WLSH1_k127_7741859_0
Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)
Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine
K04075
-
6.3.4.19
0.0000000000000000000000001146
109.0
WLSH1_k127_7753767_1
RNHCP domain
-
-
-
0.0000000000000000000008278
96.0
WLSH1_k127_7753767_2
Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine
K04075
-
6.3.4.19
0.000000000007843
67.0
WLSH1_k127_7826617_0
NAD( ) synthase glutamine-hydrolyzing
K01950
-
6.3.5.1
0.000000000000000000001639
100.0
WLSH1_k127_7826617_1
Belongs to the Nudix hydrolase family
K01515
-
3.6.1.13
0.000000000000000000278
96.0
WLSH1_k127_7826617_2
Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP
K00858
-
2.7.1.23
0.0000000000007879
72.0
WLSH1_k127_7826617_3
Catalyzes the reversible adenylation of nicotinate mononucleotide (NaMN) to nicotinic acid adenine dinucleotide (NaAD)
K00969
-
2.7.7.18
0.00000000000142
75.0
WLSH1_k127_7826617_4
Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP
K00858
-
2.7.1.23
0.00003149
46.0
WLSH1_k127_7826617_5
GDP-mannose mannosyl hydrolase activity
K03574
-
3.6.1.55
0.0005679
45.0
WLSH1_k127_7829852_0
One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit
Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine-Dalgarno cleft in the 70S ribosome
Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex
K02115
-
-
0.00000000000000000000000000000000004161
143.0
WLSH1_k127_786396_3
it plays a direct role in the translocation of protons across the membrane
K02108
-
-
0.00000000000000007492
88.0
WLSH1_k127_786396_4
F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation
Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex
Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family
-
-
-
0.000000000000000000000000002256
127.0
WLSH1_k127_7869859_1
Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family
K18989
-
-
0.00000000000000000001117
99.0
WLSH1_k127_7869859_2
Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family
-
-
-
0.00000001436
58.0
WLSH1_k127_7869859_3
Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family
K03296
-
-
0.0000000179
59.0
WLSH1_k127_7869859_4
Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family
K18989
-
-
0.00006551
49.0
WLSH1_k127_7876006_0
damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage
IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins
Non-canonical ABC transporter that contains transmembrane domains (TMD), which form a pore in the membrane, and an ATP-binding domain (NBD), which is responsible for energy generation. Confers resistance against macrolides
ABC-type antimicrobial peptide transport system, permease component
K02004
-
-
0.000000000000000000000000000000006757
136.0
WLSH1_k127_7918928_2
Efflux ABC transporter permease protein
K02004
-
-
0.00000000000000000000000000001033
123.0
WLSH1_k127_7918928_3
Negative regulator of class I heat shock genes (grpE- dnaK-dnaJ and groELS operons). Prevents heat-shock induction of these operons
K03705
-
-
0.0000000000000000000000000445
117.0
WLSH1_k127_7918928_4
Negative regulator of class I heat shock genes (grpE- dnaK-dnaJ and groELS operons). Prevents heat-shock induction of these operons
K03705
-
-
0.0006667
44.0
WLSH1_k127_7922183_0
One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Required for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA
This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis
K02358
-
-
1.208e-195
616.0
WLSH1_k127_7983964_1
Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome
The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision
The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision
K03703
-
-
0.000000000000001184
81.0
WLSH1_k127_8060934_3
The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis
The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision
K03703
-
-
0.0005322
49.0
WLSH1_k127_8108874_0
Belongs to the thioredoxin family
K03671
-
-
0.0009684
46.0
WLSH1_k127_8177559_0
Phage-related minor tail protein
-
-
-
0.0001844
55.0
WLSH1_k127_8288276_0
Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family
Involved in the restart of stalled replication forks. Recognizes and binds the arrested nascent DNA chain at stalled replication forks. It can open the DNA duplex, via its helicase activity, and promote assembly of the primosome and loading of the major replicative helicase DnaB onto DNA
Negatively regulates transcription of bacterial ribonucleotide reductase nrd genes and operons by binding to NrdR- boxes
K07738
-
-
0.00000000000000000000000000000000009997
138.0
WLSH1_k127_8597853_2
N-acetylmuramoyl-L-alanine amidase
-
-
-
0.00000000000000000000000004755
122.0
WLSH1_k127_8597853_3
Stage II sporulation protein
K06381
-
-
0.00000000005502
68.0
WLSH1_k127_8597853_4
-
-
-
-
0.000001232
54.0
WLSH1_k127_8597853_5
NYN domain
-
-
-
0.000002015
55.0
WLSH1_k127_8635064_0
Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2- cyclopenten-1-yl)amino)methyl)-7-deazaguanosine)
Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2- cyclopenten-1-yl)amino)methyl)-7-deazaguanosine)
K00773
-
2.4.2.29
0.000000000000000000000000002108
113.0
WLSH1_k127_8635064_2
Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2- cyclopenten-1-yl)amino)methyl)-7-deazaguanosine)
Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA
K03074
-
-
0.0000000000000000000000000000000000000003173
162.0
WLSH1_k127_8651140_1
NADPH-quinone reductase (modulator of drug activity B)
K00355
-
1.6.5.2
0.0000000000000000000000001464
113.0
WLSH1_k127_8651140_2
multi-organism process
-
-
-
0.00000000000004402
78.0
WLSH1_k127_8651140_3
Phosphoribosyl-ATP pyrophosphohydrolase
-
-
-
0.0000000001038
65.0
WLSH1_k127_8651140_4
Zn-dependent hydrolases of the beta-lactamase fold
Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity
Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5- dihydroxy-2,3-pentadione (DPD)
Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis
K00759
-
2.4.2.7
0.0000000000000000000000000000000000000011
150.0
WLSH1_k127_8773470_2
Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5- dihydroxy-2,3-pentadione (DPD)
K07173
-
4.4.1.21
0.0000000000000000002208
87.0
WLSH1_k127_8777986_0
helicase activity
-
-
-
0.000000000000001497
77.0
WLSH1_k127_8807149_0
Methyl-accepting chemotaxis protein
K03406
-
-
0.000000000000000000000000000000000000000002366
159.0
WLSH1_k127_8807149_1
Adenylyl- / guanylyl cyclase, catalytic domain
K01768
-
4.6.1.1
0.00000000000000002697
84.0
WLSH1_k127_8807149_2
COG2114 Adenylate cyclase, family 3 (some proteins contain HAMP domain)
K01768
-
4.6.1.1
0.0000000000000006341
83.0
WLSH1_k127_8807149_3
CHASE2 domain
K01768
-
4.6.1.1
0.000001013
52.0
WLSH1_k127_8807149_4
Adenylate cyclase
K01768
-
4.6.1.1
0.00001096
50.0
WLSH1_k127_8851528_0
PFAM membrane protein of
K08972
-
-
0.00001016
52.0
WLSH1_k127_8851528_2
An RNase that has 5'-3' exonuclease and possibly endonuclease activity. Involved in maturation of rRNA and in some organisms also mRNA maturation and or decay
K12574
-
-
0.0001227
51.0
WLSH1_k127_8858276_0
DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity
DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity
K02343
-
2.7.7.7
0.0002875
51.0
WLSH1_k127_8870381_0
Responsible for synthesis of pseudouridine from uracil
Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity
K03531
-
-
0.000000000006926
68.0
WLSH1_k127_9315672_1
PFAM Cold-shock protein, DNA-binding
K03704
-
-
0.00001003
51.0
WLSH1_k127_9347346_0
PFAM peptidase M3A and M3B thimet oligopeptidase F
Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses L-glutamine as a nitrogen source
K01950
-
6.3.5.1
0.000000000000000000000002863
105.0
WLSH1_k127_9399444_3
NUDIX domain
-
-
-
0.00000000000000001518
83.0
WLSH1_k127_9399444_4
NUDIX domain
-
-
-
0.0000000000000000477
81.0
WLSH1_k127_9422044_0
Transcriptional regulator, TrmB
-
-
-
0.0000000000002244
76.0
WLSH1_k127_9422044_1
PHP domain protein
K07053
-
3.1.3.97
0.00004389
46.0
WLSH1_k127_9520733_0
One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex
Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family
K03296
-
-
0.0000000000000000000000000008508
125.0
WLSH1_k127_9563672_0
Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-2 is significantly reduced by GTP and GDP, but not by GMP
Required for disulfide bond formation in some periplasmic proteins. Acts by transferring its disulfide bond to other proteins and is reduced in the process
-
-
-
0.0000000000000000000000000000000006448
138.0
WLSH1_k127_9638411_1
IMP dehydrogenase / GMP reductase domain
K00088,K00364
-
1.1.1.205,1.7.1.7
0.00001049
50.0
WLSH1_k127_9639229_0
Belongs to the anti-sigma-factor antagonist family
K06378
-
-
0.00000509
53.0
WLSH1_k127_9639229_1
FemAB family
-
-
-
0.00001048
55.0
WLSH1_k127_9639229_2
peptidase
-
-
-
0.0009656
49.0
WLSH1_k127_9648563_0
His Kinase A (phospho-acceptor) domain
K03406
-
-
0.000000000000000000000000000000001082
143.0
WLSH1_k127_9674172_0
Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides
K00526
-
1.17.4.1
0.00000000000000000000000000000000000000001086
158.0
WLSH1_k127_9674172_1
Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis
K00287
-
1.5.1.3
0.00000000000000000000000001979
111.0
WLSH1_k127_9674172_2
Dihydrofolate reductase
K00287
-
1.5.1.3
0.00007138
47.0
WLSH1_k127_9780233_0
Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner
Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins
One of the essential components for the initiation of protein synthesis. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps modulate mRNA selection, yielding the 30S pre- initiation complex (PIC). Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are released leaving the mature 70S translation initation complex
