Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA
Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA
Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides
Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides
Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates
Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln)
K02433
GO:0008150,GO:0040007
6.3.5.6,6.3.5.7
0.0000000000001388
71.0
WLSH2_k127_10386052_0
Binds directly to 23S rRNA. The L1 stalk is quite mobile in the ribosome, and is involved in E site tRNA release
transferase activity, transferring acyl groups other than amino-acyl groups
-
-
-
0.00000000000000000000000000000000000000000001903
175.0
WLSH2_k127_10496462_1
Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D- arabino-heptulosonate-7-phosphate (DAHP)
K01626
-
2.5.1.54
0.000003812
51.0
WLSH2_k127_1060729_0
Cytosine-specific methyltransferase
K00558
-
2.1.1.37
0.0000000000001209
74.0
WLSH2_k127_1060729_1
Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family
TIGRFAM glycosyl transferase, WecB TagA CpsF family
K13660
-
-
0.00002748
51.0
WLSH2_k127_11022875_0
Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA
COGs COG5001 signal transduction protein containing a membrane domain an EAL and a GGDEF domain
-
-
-
0.0007044
46.0
WLSH2_k127_11356183_0
PFAM HI0933-like protein
K07007
-
-
0.000000000000000000000000000000000004229
148.0
WLSH2_k127_11356183_1
Flavoprotein family
K07007
-
-
0.000000000000009713
76.0
WLSH2_k127_11384422_0
Ribonuclease
K12573
-
-
0.0000000002365
64.0
WLSH2_k127_11462864_0
First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan
K01000
-
2.7.8.13
0.000000000000000000000000000000009154
132.0
WLSH2_k127_11462864_1
First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan
K01000
-
2.7.8.13
0.0000000000000004263
85.0
WLSH2_k127_11565137_0
Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate- limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth
K00088
-
1.1.1.205
0.00002995
55.0
WLSH2_k127_117085_0
The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis
The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by UvrB, the UvrA molecules dissociate
Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan
Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan
K01928
-
6.3.2.13
0.0000000000000000000002196
99.0
WLSH2_k127_1320622_0
Critical role in recombination and DNA repair. Helps process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3'- to 5'- polarity. Unwinds branched duplex DNA (Y-DNA)
K03655
-
3.6.4.12
0.00000000000000000000000000001414
128.0
WLSH2_k127_133770_0
Sigma-70, region 4
K03088
-
-
0.00000000000000006976
83.0
WLSH2_k127_133770_1
ECF sigma factor
K03088
-
-
0.0000000000006248
72.0
WLSH2_k127_133770_2
Uncharacterized protein domain (DUF2202)
-
-
-
0.000000000008638
70.0
WLSH2_k127_133770_3
Belongs to the short-chain dehydrogenases reductases (SDR) family
K14189
-
-
0.0000000004695
60.0
WLSH2_k127_133770_4
-
-
-
-
0.000000003243
63.0
WLSH2_k127_133770_5
Uncharacterized protein domain (DUF2202)
-
-
-
0.000003562
53.0
WLSH2_k127_1383502_0
peptidyl-tyrosine sulfation
-
-
-
0.00000001556
60.0
WLSH2_k127_1383502_1
TIGRFAM DNA polymerase III, delta subunit
K02340
-
2.7.7.7
0.0000008499
57.0
WLSH2_k127_1383502_2
DNA polymerase III subunit delta
K02340
-
2.7.7.7
0.00006391
50.0
WLSH2_k127_1447611_0
DNA-dependent ATPase involved in processing of recombination intermediates, plays a role in repairing DNA breaks. Stimulates the branch migration of RecA-mediated strand transfer reactions, allowing the 3' invading strand to extend heteroduplex DNA faster. Binds ssDNA in the presence of ADP but not other nucleotides, has ATPase activity that is stimulated by ssDNA and various branched DNA structures, but inhibited by SSB. Does not have RecA's homology-searching function
DNA-dependent ATPase involved in processing of recombination intermediates, plays a role in repairing DNA breaks. Stimulates the branch migration of RecA-mediated strand transfer reactions, allowing the 3' invading strand to extend heteroduplex DNA faster. Binds ssDNA in the presence of ADP but not other nucleotides, has ATPase activity that is stimulated by ssDNA and various branched DNA structures, but inhibited by SSB. Does not have RecA's homology-searching function
K04485
-
-
0.0000000001329
64.0
WLSH2_k127_1454334_0
PFAM PKD domain containing protein
-
-
-
0.0000000000000000000002139
113.0
WLSH2_k127_1483178_0
general secretion pathway protein G
K02456
-
-
0.0004488
47.0
WLSH2_k127_1483572_0
2-oxobutyrate metabolic process
K01733
-
4.2.3.1
0.00000000000000000000001146
106.0
WLSH2_k127_1494375_0
Protein of unknown function (DUF1624)
-
-
-
0.00000000006344
68.0
WLSH2_k127_1494375_1
Chlorophyllase
K01061
-
3.1.1.45
0.000000006312
57.0
WLSH2_k127_1494375_2
dienelactone hydrolase
K01061
-
3.1.1.45
0.000000009729
57.0
WLSH2_k127_1494375_3
Dienelactone hydrolase
K01061
-
3.1.1.45
0.00001502
48.0
WLSH2_k127_1494375_4
BAAT / Acyl-CoA thioester hydrolase C terminal
-
-
-
0.0003443
44.0
WLSH2_k127_1513746_0
Possible plasma membrane-binding motif in junctophilins, PIP-5-kinases and protein kinases.
-
-
-
0.000000008022
64.0
WLSH2_k127_1518860_0
Metal dependent phosphohydrolases with conserved 'HD' motif.
-
-
-
0.00000000009717
64.0
WLSH2_k127_1547934_0
Provides the (R)-glutamate required for cell wall biosynthesis
K01776
-
5.1.1.3
0.00000000009717
64.0
WLSH2_k127_1547934_1
Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)
Critical role in recombination and DNA repair. Helps process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3'- to 5'- polarity. Unwinds branched duplex DNA (Y-DNA)
COG0719 ABC-type transport system involved in Fe-S cluster assembly, permease component
K09014
-
-
0.00000000000001192
74.0
WLSH2_k127_1687285_2
Uncharacterized protein family (UPF0051)
K07033
-
-
0.00000000005195
67.0
WLSH2_k127_1687857_0
A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner
Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates
K01937
-
6.3.4.2
0.000000000000000000000000000000000009481
137.0
WLSH2_k127_1687857_2
Nuclease-related domain
-
-
-
0.000000003673
57.0
WLSH2_k127_1735128_0
Involved in molybdopterin and thiamine biosynthesis, family 2
K21029
-
2.7.7.80
0.0000000000000000000000000000002136
134.0
WLSH2_k127_1735128_1
Succinylglutamate desuccinylase / Aspartoacylase family
-
-
-
0.000000002217
63.0
WLSH2_k127_1735128_2
Succinylglutamate desuccinylase / Aspartoacylase family
-
-
-
0.0006651
43.0
WLSH2_k127_1738676_0
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates
This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly
K02871
-
-
0.0000000000000000000000000000000000000003196
153.0
WLSH2_k127_1738676_2
Ribosomal protein S9/S16
K02996
-
-
0.00000000000000000000000000001688
121.0
WLSH2_k127_1738676_3
ribosomal protein l17
K02879
-
-
0.0000000000000001724
83.0
WLSH2_k127_1870750_0
Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria
Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria
K02338
-
2.7.7.7
0.00000374
53.0
WLSH2_k127_1898496_0
Ribosomal RNA adenine dimethylase
-
-
-
0.0000000000000003697
80.0
WLSH2_k127_1968226_0
Phosphoglycerate mutase family
-
-
-
0.000000000000000000000000000000000008338
143.0
WLSH2_k127_1968226_2
nUDIX hydrolase
K07006
GO:0003674,GO:0003824,GO:0016787
-
0.00000057
53.0
WLSH2_k127_1968226_3
nUDIX hydrolase
K07006
GO:0003674,GO:0003824,GO:0016787
-
0.0003638
46.0
WLSH2_k127_1986133_0
Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein
K01929
-
6.3.2.10
0.000000000004889
73.0
WLSH2_k127_2047425_0
PFAM membrane protein of
K08972
-
-
0.00001016
52.0
WLSH2_k127_2209657_0
General secretory system II protein E domain protein
COG2804 Type II secretory pathway, ATPase PulE Tfp pilus assembly pathway, ATPase PilB
K02243,K02652
-
-
0.00000000000000000000000000000000003952
138.0
WLSH2_k127_2222790_0
it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP
K03629
-
-
0.00000000000000000000000004546
117.0
WLSH2_k127_2222790_1
Tyrosine recombinase XerD
K04763
-
-
0.000000000000000000000003718
104.0
WLSH2_k127_2222790_2
it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP
K03629
-
-
0.0000001105
55.0
WLSH2_k127_2222790_3
Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln)
K02435
-
6.3.5.6,6.3.5.7
0.00003428
49.0
WLSH2_k127_2222790_4
domain protein
K07004,K09955,K16915,K20276
-
-
0.00004291
56.0
WLSH2_k127_2233260_0
domain protein
-
-
-
0.00005475
54.0
WLSH2_k127_2379847_0
Functions as a peptidoglycan terminase that cleaves nascent peptidoglycan strands endolytically to terminate their elongation
Required for disulfide bond formation in some periplasmic proteins. Acts by transferring its disulfide bond to other proteins and is reduced in the process
One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity
One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit
K02906
-
-
0.000000000000000000000000000000004528
134.0
WLSH2_k127_2573110_3
One of the early assembly proteins it binds 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome. Forms the main docking site for trigger factor binding to the ribosome
K02892
-
-
0.000000000006809
69.0
WLSH2_k127_2573110_4
Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA
One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity
ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins
K03686
-
-
0.0001414
46.0
WLSH2_k127_2628853_0
NYN domain
-
-
-
0.000002015
55.0
WLSH2_k127_2674916_0
Catalyzes the reductive methylation of 2'-deoxyuridine- 5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis
Catalyzes the reductive methylation of 2'-deoxyuridine- 5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis
K00560
-
2.1.1.45
0.00007093
47.0
WLSH2_k127_2674916_5
Bacterial PH domain
K08981
-
-
0.0007889
49.0
WLSH2_k127_2677428_0
Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)
Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins
Catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L- selenocystine to produce L-alanine
K11717
-
2.8.1.7,4.4.1.16
0.0000000000000000000000008199
108.0
WLSH2_k127_2677428_3
Catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L- selenocystine to produce L-alanine
K11717
-
2.8.1.7,4.4.1.16
0.00000000000000000002889
93.0
WLSH2_k127_2688594_0
4Fe-4S single cluster domain
-
-
-
0.0000000000000000000000000000000000000000004511
162.0
WLSH2_k127_2688594_1
4Fe-4S single cluster domain
-
-
-
0.0000000000000005807
81.0
WLSH2_k127_2688594_2
4Fe-4S single cluster domain
-
-
-
0.000000000000002413
80.0
WLSH2_k127_2697294_0
Mu-like prophage FluMu protein gp28
-
-
-
0.0008796
48.0
WLSH2_k127_2715705_0
metallopeptidase activity
-
-
-
0.00003883
53.0
WLSH2_k127_2749881_0
R3H domain
K06346
-
-
0.000000753
58.0
WLSH2_k127_2749881_1
peptidyl-tyrosine sulfation
-
-
-
0.00004934
54.0
WLSH2_k127_2757318_0
Participates in initiation and elongation during chromosome replication
K02314
-
3.6.4.12
0.0000000000000000000002113
98.0
WLSH2_k127_2804444_0
cell adhesion involved in biofilm formation
-
-
-
0.000001441
60.0
WLSH2_k127_2811973_0
Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp Asn)
Metal dependent phosphohydrolases with conserved 'HD' motif.
K06950
-
-
0.00000000000000000000000000000000000004637
145.0
WLSH2_k127_2811973_2
Metal dependent phosphohydrolases with conserved 'HD' motif.
K06950
-
-
0.0005298
45.0
WLSH2_k127_2812282_0
PFAM metal-dependent phosphohydrolase, HD sub domain
K02030
-
-
0.0000003102
57.0
WLSH2_k127_2815689_0
Endonuclease Exonuclease Phosphatase
-
-
-
0.000000000000000000000000005427
118.0
WLSH2_k127_284741_0
Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase
Belongs to the class-I aminoacyl-tRNA synthetase family
K01883
-
6.1.1.16
0.000000000000000001707
87.0
WLSH2_k127_2883965_0
Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family
-
-
-
0.000000000000000000000000002256
127.0
WLSH2_k127_2883965_1
Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family
-
-
-
0.00000000000002745
76.0
WLSH2_k127_2883965_2
Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family
K18989
-
-
0.000000000002113
72.0
WLSH2_k127_2883965_3
Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family
K03296
-
-
0.0000000179
59.0
WLSH2_k127_2883965_4
Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family
K18989
-
-
0.00006551
49.0
WLSH2_k127_2929404_0
Reversibly transfers an adenylyl group from ATP to 4'- phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate
K00954
-
2.7.7.3
0.00000000000008637
76.0
WLSH2_k127_2929404_1
Isochorismatase family
K08281
-
3.5.1.19
0.00000004144
59.0
WLSH2_k127_2933354_0
sensory perception of sound
-
-
-
0.00004781
50.0
WLSH2_k127_2933354_1
Lytic transglycosylase with a strong preference for naked glycan strands that lack stem peptides
K03642
-
-
0.0003189
45.0
WLSH2_k127_2933354_2
Cysteine-rich secretory protein family
-
-
-
0.0008264
43.0
WLSH2_k127_2933461_0
In addition to polymerase activity, this DNA polymerase exhibits 5'-3' exonuclease activity
K02335
-
2.7.7.7
0.000000000000000000000000000000000000006683
149.0
WLSH2_k127_2933461_1
In addition to polymerase activity, this DNA polymerase exhibits 5'-3' exonuclease activity
K02335
-
2.7.7.7
0.0000000000000000000000000002262
117.0
WLSH2_k127_2933461_2
In addition to polymerase activity, this DNA polymerase exhibits 5'-3' exonuclease activity
K02335
-
2.7.7.7
0.0002962
43.0
WLSH2_k127_2934305_0
Belongs to the anti-sigma-factor antagonist family
K06378
-
-
0.00000509
53.0
WLSH2_k127_308156_0
amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile)
amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile)
Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate
Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit
One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, probably blocks exit of the E-site tRNA
K02992
-
-
0.00000000000000000000000000000000002391
139.0
WLSH2_k127_3350148_2
Belongs to the bacterial ribosomal protein bL27 family
Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate
Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF- independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism
Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate
K01491
-
1.5.1.5,3.5.4.9
0.0000000000006473
69.0
WLSH2_k127_3582636_5
TIGRFAM DNA internalization-related competence protein ComEC Rec2
K02238
-
-
0.000000000001253
78.0
WLSH2_k127_3582636_6
Couples transcription and DNA repair by recognizing RNA polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent release of RNAP and its truncated transcript from the DNA, and recruitment of nucleotide excision repair machinery to the damaged site
Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another
K02838
-
-
0.00000000000000000000000000000000000000000002053
168.0
WLSH2_k127_3644574_0
COGs COG4339 conserved
-
-
-
0.00000000000000000001175
101.0
WLSH2_k127_3688121_0
Penicillin-binding Protein dimerisation domain
K03587
-
3.4.16.4
0.0000000000001414
73.0
WLSH2_k127_3688121_1
Penicillin-binding Protein dimerisation domain
K03587
-
3.4.16.4
0.000000000004243
68.0
WLSH2_k127_3688121_2
Penicillin-binding protein, transpeptidase domain protein
One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex
Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ
K03687
-
-
0.0000000000000000000001064
104.0
WLSH2_k127_3889318_0
PFAM single-strand binding protein Primosomal replication protein n
K03111
-
-
0.00000000000000000000000000000000006297
138.0
WLSH2_k127_389220_0
Psort location Cytoplasmic, score
-
-
-
0.00000002085
59.0
WLSH2_k127_389220_1
Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria
K02338
-
2.7.7.7
0.00004167
49.0
WLSH2_k127_3902611_0
Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage
Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage
Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec)
K01875
-
6.1.1.11
0.000000000000000000000000000000000000006865
149.0
WLSH2_k127_3902611_3
Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec)
K01875
-
6.1.1.11
0.00000000000000000000000000003117
122.0
WLSH2_k127_3902611_4
nitric oxide dioxygenase activity
K00523,K02823
-
1.17.1.1
0.000000000000000000009408
100.0
WLSH2_k127_3913718_0
Type II/IV secretion system protein
K02669
-
-
0.000006772
52.0
WLSH2_k127_3944110_0
Involved in transcription antitermination. Required for transcription of ribosomal RNA (rRNA) genes. Binds specifically to the boxA antiterminator sequence of the ribosomal RNA (rrn) operons
Single strand-specific metallo-endoribonuclease involved in late-stage 70S ribosome quality control and in maturation of the 3' terminus of the 16S rRNA
K01489,K07042
-
3.5.4.5
0.0000000000000000000004407
101.0
WLSH2_k127_3988773_1
Major Facilitator Superfamily
-
-
-
0.00000263
54.0
WLSH2_k127_4053031_0
GTPase that plays an essential role in the late steps of ribosome biogenesis
K03977
-
-
0.00000000000000000000000000000009281
130.0
WLSH2_k127_4053031_1
DUF167
K09131
-
-
0.0002798
45.0
WLSH2_k127_4091258_0
ATPase that binds to both the 70S ribosome and the 50S ribosomal subunit in a nucleotide-independent manner
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction
K01409
-
2.3.1.234
0.000000000000000001491
88.0
WLSH2_k127_4208620_2
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction
K01409
-
2.3.1.234
0.0001485
45.0
WLSH2_k127_4283997_0
Diguanylate cyclase
-
-
-
0.00000000000000000000000008409
112.0
WLSH2_k127_4283997_1
Putative diguanylate phosphodiesterase
-
-
-
0.000000001939
64.0
WLSH2_k127_4283997_2
EAL domain protein
-
-
-
0.00001288
48.0
WLSH2_k127_4283997_3
PFAM EAL domain
-
-
-
0.0001385
46.0
WLSH2_k127_4292068_0
PFAM Transposase DDE domain
-
-
-
0.00001112
54.0
WLSH2_k127_4292068_1
PFAM Transposase DDE domain
-
-
-
0.00002358
49.0
WLSH2_k127_4312362_0
The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB
Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins
An essential GTPase that binds both GDP and GTP, with rapid nucleotide exchange. Plays a role in 16S rRNA processing and 30S ribosomal subunit biogenesis and possibly also in cell cycle regulation and energy metabolism
K03595
-
-
0.0000000000000000000000004098
109.0
WLSH2_k127_447731_0
Mediates influx of magnesium ions
K03284
-
-
0.00000000000000000000000000000000001692
147.0
WLSH2_k127_447731_1
Could be involved in insertion of integral membrane proteins into the membrane
K08998
-
-
0.000000000000000000002249
95.0
WLSH2_k127_4487549_0
Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)
Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)
Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol
-
-
-
0.000000000000000000162
93.0
WLSH2_k127_4630733_1
Phospholipase D. Active site motifs.
-
-
-
0.0008684
44.0
WLSH2_k127_4658158_0
Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone
K03168
-
5.99.1.2
0.00000000000000000000000000000000000002884
151.0
WLSH2_k127_4658158_1
multi-organism process
-
-
-
0.00000000000000000000000000000000000009559
147.0
WLSH2_k127_4658158_2
Type IV secretory pathway, VirB4
-
-
-
0.0000000000000000000000005322
110.0
WLSH2_k127_4658158_3
PFAM Stage II sporulation E family protein
K07315
-
3.1.3.3
0.000000000000000000001582
102.0
WLSH2_k127_4658158_4
Type IV secretory pathway, VirB4
-
-
-
0.000000000000006501
83.0
WLSH2_k127_4658158_5
S4 domain
K14761
-
-
0.000000001814
62.0
WLSH2_k127_4658158_6
COG COG3451 Type IV secretory pathway, VirB4 components
-
-
-
0.000000005262
59.0
WLSH2_k127_4669754_0
Mg chelatase-like protein
K07391
-
-
0.000000000000000000000000000000000000000004216
159.0
WLSH2_k127_4675246_0
In addition to polymerase activity, this DNA polymerase exhibits 5'-3' exonuclease activity
An RNase that has 5'-3' exonuclease and possibly endonuclease activity. Involved in maturation of rRNA and in some organisms also mRNA maturation and or decay
Belongs to the ribose-phosphate pyrophosphokinase family
-
-
-
0.0000000006458
60.0
WLSH2_k127_4716533_5
Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis
K00759
-
2.4.2.7
0.0000000158
64.0
WLSH2_k127_4716533_6
Belongs to the ribose-phosphate pyrophosphokinase family
-
-
-
0.0003507
45.0
WLSH2_k127_4725598_0
Sigma-70, region 4
K03086
-
-
0.000000009692
64.0
WLSH2_k127_4783024_0
Type II secretory pathway, ATPase PulE Tfp pilus assembly pathway, ATPase PilB
K02454,K02652
-
-
0.00000000000000000000000000002416
121.0
WLSH2_k127_4792977_0
A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner
K02469
-
5.99.1.3
8.479e-245
782.0
WLSH2_k127_4792977_1
Carrier of the growing fatty acid chain in fatty acid biosynthesis
K02078
-
-
0.000607
47.0
WLSH2_k127_4824634_0
Nicotinate phosphoribosyltransferase (NAPRTase) family
K03462
-
2.4.2.12
0.000004053
57.0
WLSH2_k127_484017_0
Large extracellular alpha-helical protein
K06894
-
-
0.000000000000000000000001618
112.0
WLSH2_k127_484017_1
PFAM alpha-2-macroglobulin domain protein
K06894
-
-
0.000000000000006341
83.0
WLSH2_k127_4859357_0
Psort location Cytoplasmic, score 8.96
K04096
-
-
0.000000000000000000000000000005322
124.0
WLSH2_k127_4859357_1
DNA protecting protein DprA
K04096
-
-
0.0000000000000000000000000002004
122.0
WLSH2_k127_4859357_2
Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine
K04075
-
6.3.4.19
0.000000000000000000000000295
111.0
WLSH2_k127_4859357_3
RNHCP domain
-
-
-
0.0000000000000000000008278
96.0
WLSH2_k127_4859357_4
pathway protein E
K02454
-
-
0.000000000000007002
79.0
WLSH2_k127_4859357_5
Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine
K04075
-
6.3.4.19
0.000000000007843
67.0
WLSH2_k127_4906716_0
Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34
In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance
amino acids such as threonine, to avoid such errors, it has a posttransfer editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner
Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth
Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys- tRNA(Pro) is not edited by ProRS
Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)
Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)
K01881
-
6.1.1.15
0.00000000002167
68.0
WLSH2_k127_5327827_4
Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)
Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation
Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines
Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines
K05540
-
-
0.000000000001084
72.0
WLSH2_k127_5571748_0
Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome
Transfers the N-acyl diglyceride group on what will become the N-terminal cysteine of membrane lipoproteins
K13292
-
-
0.00000005209
63.0
WLSH2_k127_565297_0
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction
K01409
-
2.3.1.234
0.0000000000000000000000000006391
116.0
WLSH2_k127_565297_1
cell division
-
-
-
0.0000000000005578
78.0
WLSH2_k127_565297_2
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction
PFAM L-carnitine dehydratase bile acid-inducible protein F
K18702
-
2.8.3.19
0.000000000000000000000000000000003507
132.0
WLSH2_k127_5928810_1
CoA-transferase family III
K18702
-
2.8.3.19
0.00000000000003038
74.0
WLSH2_k127_5928810_2
Part of the MsrPQ system that repairs oxidized periplasmic proteins containing methionine sulfoxide residues (Met-O), using respiratory chain electrons. Thus protects these proteins from oxidative-stress damage caused by reactive species of oxygen and chlorine generated by the host defense mechanisms. MsrPQ is essential for the maintenance of envelope integrity under bleach stress, rescuing a wide series of structurally unrelated periplasmic proteins from methionine oxidation. MsrQ provides electrons for reduction to the reductase catalytic subunit MsrP, using the quinone pool of the respiratory chain
K17247
-
-
0.00000003169
63.0
WLSH2_k127_6053983_0
amino acids such as threonine, to avoid such errors, it has a posttransfer editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner
Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits
K02952
-
-
0.000000000000000000000000000000000000001723
149.0
WLSH2_k127_6185591_3
Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine-Dalgarno cleft in the 70S ribosome
One of the essential components for the initiation of protein synthesis. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps modulate mRNA selection, yielding the 30S pre- initiation complex (PIC). Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are released leaving the mature 70S translation initation complex
An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. Plays a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control
Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions
K03530
-
-
0.000000001003
61.0
WLSH2_k127_6240423_0
Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain
Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine
K03648
-
3.2.2.27
0.00000000000000000000000000000002191
126.0
WLSH2_k127_6240423_2
Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group
K01159
-
3.1.22.4
0.000000000000000001403
89.0
WLSH2_k127_6240423_3
Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine
K03648
-
3.2.2.27
0.0000000000000000161
81.0
WLSH2_k127_6240423_4
Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group
Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine
The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently
This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7 L12 stalk, and near the tRNA binding site of the peptidyltransferase center
Located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body
K02988
-
-
0.000000000000000000000000000000000003804
146.0
WLSH2_k127_6340495_3
This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance
One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit
Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan
K01928
-
6.3.2.13
0.00000000000000000000000000000000000000003334
158.0
WLSH2_k127_6397676_0
The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing
Negatively regulates transcription of bacterial ribonucleotide reductase nrd genes and operons by binding to NrdR- boxes
K07738
-
-
0.00000000000000000000007397
100.0
WLSH2_k127_6443290_1
Stage II sporulation protein
K06381
-
-
0.00000000005502
68.0
WLSH2_k127_6443290_2
-
-
-
-
0.000001232
54.0
WLSH2_k127_6443290_3
Animal peptidoglycan recognition proteins homologous to Bacteriophage T3 lysozyme.
-
-
-
0.00001818
50.0
WLSH2_k127_6447247_0
Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits
K02528
-
2.1.1.182
0.00000000000000000001835
95.0
WLSH2_k127_6447247_1
Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits
Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP
K00858
-
2.7.1.23
0.0000000000007879
72.0
WLSH2_k127_6455489_5
Catalyzes the reversible adenylation of nicotinate mononucleotide (NaMN) to nicotinic acid adenine dinucleotide (NaAD)
K00969
-
2.7.7.18
0.00000000000142
75.0
WLSH2_k127_6455489_6
Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP
Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner
Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner
K03596
-
-
0.00000000002032
64.0
WLSH2_k127_6530342_0
Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family
Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P)
General secretory system II, protein E domain protein
K02454,K02652
-
-
0.0001278
45.0
WLSH2_k127_6777926_0
Belongs to the peptidase S41A family
K03797
-
3.4.21.102
0.000000000000000002486
88.0
WLSH2_k127_6777926_1
tail specific protease
K03797
-
3.4.21.102
0.0000000000001221
78.0
WLSH2_k127_6777926_2
PFAM Type II secretion system F domain
K02653
-
-
0.0000000000002225
72.0
WLSH2_k127_6777926_3
tail specific protease
K03797
-
3.4.21.102
0.000000001448
60.0
WLSH2_k127_6777926_4
Belongs to the peptidase S41A family
K03797
-
3.4.21.102
0.0000007238
51.0
WLSH2_k127_6777926_5
Chaperone of endosialidase
-
-
-
0.0001959
44.0
WLSH2_k127_6828744_0
Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln)
Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln)
K02433
GO:0008150,GO:0040007
6.3.5.6,6.3.5.7
0.0004857
43.0
WLSH2_k127_6834539_0
Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome
RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication
K02316
-
-
0.000000000000000000000000002044
116.0
WLSH2_k127_7061091_1
COG0500 SAM-dependent methyltransferases
-
-
-
0.000000000000196
79.0
WLSH2_k127_7061091_2
2 iron, 2 sulfur cluster binding
K13771
-
-
0.000001576
51.0
WLSH2_k127_7105595_0
Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2- cyclopenten-1-yl)amino)methyl)-7-deazaguanosine)
Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2- cyclopenten-1-yl)amino)methyl)-7-deazaguanosine)
K00773
-
2.4.2.29
0.000000000000000000000000002108
113.0
WLSH2_k127_7105595_2
Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2- cyclopenten-1-yl)amino)methyl)-7-deazaguanosine)
Cytochrome C biogenesis protein transmembrane region
-
-
-
0.00000000000000000000000000000004859
134.0
WLSH2_k127_714146_2
ATPase, P-type (transporting), HAD superfamily, subfamily IC
K17686
-
3.6.3.54
0.000000000000000000000004478
102.0
WLSH2_k127_714146_3
Cytochrome C biogenesis protein transmembrane region
-
-
-
0.000000000000000000003298
99.0
WLSH2_k127_714146_4
Metal-sensitive transcriptional repressor
K21600
-
-
0.000000000001156
71.0
WLSH2_k127_714146_5
Heavy metal transport detoxification protein
-
-
-
0.000000000001299
70.0
WLSH2_k127_714146_6
Heavy-metal-associated domain
K17686
-
3.6.3.54
0.000000007438
57.0
WLSH2_k127_714146_7
Short C-terminal domain
K08982
-
-
0.00000002536
60.0
WLSH2_k127_715716_0
Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12
K14441
-
2.8.4.4
0.0000000000000000000000000000000000000001488
160.0
WLSH2_k127_715716_1
Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln)
Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE
Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE
Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane
Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving both as a receptor for the preprotein-SecB complex and as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane
K03070
-
-
0.000000000000000000000000000000000000746
141.0
WLSH2_k127_733699_2
Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving both as a receptor for the preprotein-SecB complex and as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane
K03070
-
-
0.0000000000000006331
82.0
WLSH2_k127_733699_3
Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane
Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2- cyclopenten-1-yl)amino)methyl)-7-deazaguanosine)
K00773
-
2.4.2.29
0.000000000001094
68.0
WLSH2_k127_7503655_2
Rhomboid family
-
-
-
0.00000000001282
70.0
WLSH2_k127_7511205_0
guanyl-nucleotide exchange factor activity
-
-
-
0.00000000000003564
78.0
WLSH2_k127_7511205_1
-
-
-
-
0.0000000002838
66.0
WLSH2_k127_7511205_2
regulator of chromosome condensation, RCC1
-
-
-
0.00001554
50.0
WLSH2_k127_7514919_0
DHH family
K06881
-
3.1.13.3,3.1.3.7
0.0000000000000000000000006505
111.0
WLSH2_k127_7514919_1
DHH family
K06881
-
3.1.13.3,3.1.3.7
0.0002926
46.0
WLSH2_k127_7518913_0
COGs COG1253 Hemolysins and related protein containing CBS domains
-
-
-
0.0000000000000002991
83.0
WLSH2_k127_7518913_1
Transporter associated domain
-
-
-
0.000000006751
61.0
WLSH2_k127_7518913_2
Prokaryotic N-terminal methylation motif
-
-
-
0.00002597
51.0
WLSH2_k127_752975_0
Belongs to the helicase family. UvrD subfamily
K03657
-
3.6.4.12
0.0000000000000000000000000000008561
132.0
WLSH2_k127_752975_1
Belongs to the helicase family. UvrD subfamily
K03657
-
3.6.4.12
0.00000000000000000000001627
106.0
WLSH2_k127_752975_2
Couples transcription and DNA repair by recognizing RNA polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent release of RNAP and its truncated transcript from the DNA, and recruitment of nucleotide excision repair machinery to the damaged site
K03723
-
-
0.00000009989
53.0
WLSH2_k127_7568837_0
Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5- dihydroxy-2,3-pentadione (DPD)
Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis
K00759
-
2.4.2.7
0.0000000000000000000000000000000000000011
150.0
WLSH2_k127_7568837_2
Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5- dihydroxy-2,3-pentadione (DPD)
K07173
-
4.4.1.21
0.0000000000000000002208
87.0
WLSH2_k127_7568837_3
Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S- adenosylhomocysteine (SAH AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively
K01243
-
3.2.2.9
0.00000004773
59.0
WLSH2_k127_7568837_4
Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S- adenosylhomocysteine (SAH AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively
Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)
K00791
-
2.5.1.75
0.00000000002664
64.0
WLSH2_k127_7646088_0
Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity
Part of the ABC transporter FtsEX involved in asymmetric cellular division facilitating the initiation of sporulation
K09811
-
-
0.0004734
47.0
WLSH2_k127_7730526_0
Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc- (pentapeptide)GlcNAc (lipid intermediate II)
K02563
-
2.4.1.227
0.000000004605
60.0
WLSH2_k127_7739299_0
regulation of ryanodine-sensitive calcium-release channel activity
-
-
-
0.0000005678
57.0
WLSH2_k127_7753247_0
Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family
K18302
-
-
0.0001356
48.0
WLSH2_k127_7761954_0
Glycosyl transferases group 1
-
-
-
0.00003901
51.0
WLSH2_k127_7787158_0
Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions
Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln)
Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism
This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits
K02931
-
-
0.0000000000000000000000000000000000000005319
152.0
WLSH2_k127_8359923_3
Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA
One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity
One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA
K02961
-
-
0.000000000000000001221
87.0
WLSH2_k127_8359923_7
The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome
K02890
-
-
0.00000000000000000341
88.0
WLSH2_k127_8359923_8
Binds 23S rRNA and is also seen to make contacts with the A and possibly P site tRNAs
Catalyzes hydrolysis of the D-alanyl-D-alanine dipeptide
K08641
-
3.4.13.22
0.000000000000000001705
88.0
WLSH2_k127_8379327_2
Catalyzes hydrolysis of the D-alanyl-D-alanine dipeptide
K08641
-
3.4.13.22
0.00000000000004926
74.0
WLSH2_k127_8436579_0
Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome
Catalytic histone acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation
Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA
K03438
-
2.1.1.199
0.0003676
45.0
WLSH2_k127_876543_0
Belongs to the bacterial ribosomal protein bL34 family
K02914
-
-
0.0000007504
55.0
WLSH2_k127_876543_1
RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme
Couples transcription and DNA repair by recognizing RNA polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent release of RNAP and its truncated transcript from the DNA, and recruitment of nucleotide excision repair machinery to the damaged site
Couples transcription and DNA repair by recognizing RNA polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent release of RNAP and its truncated transcript from the DNA, and recruitment of nucleotide excision repair machinery to the damaged site
K03723
-
-
0.000000000000000000000000000689
121.0
WLSH2_k127_8873031_3
Psort location Cytoplasmic, score
K00588
-
2.1.1.104
0.00000000000000000000003065
106.0
WLSH2_k127_8873031_4
Couples transcription and DNA repair by recognizing RNA polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent release of RNAP and its truncated transcript from the DNA, and recruitment of nucleotide excision repair machinery to the damaged site
K03723
-
-
0.000000000000000000009509
93.0
WLSH2_k127_8873031_5
Produces ATP from ADP in the presence of a proton gradient across the membrane
tRNA synthetase class II core domain (G, H, P, S and T)
K01892
-
6.1.1.21
0.00000005767
57.0
WLSH2_k127_8887974_0
TIGRFAM cell envelope-related function transcriptional attenuator, LytR CpsA family
-
-
-
0.0000000000000006564
87.0
WLSH2_k127_8887974_1
TRANSCRIPTIONal
-
-
-
0.0000000008194
63.0
WLSH2_k127_8887974_2
cell envelope-related transcriptional attenuator
-
-
-
0.0000009868
53.0
WLSH2_k127_8922644_0
DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA
DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA
K01972
-
6.5.1.2
0.0000002308
58.0
WLSH2_k127_8924423_0
Repeats in polycystic kidney disease 1 (PKD1) and other proteins
-
-
-
0.00008423
52.0
WLSH2_k127_8974626_0
PDZ domain (Also known as DHR or GLGF)
K08372
-
-
0.0000002234
52.0
WLSH2_k127_8974626_1
typically periplasmic contain C-terminal PDZ domain
-
GO:0003674,GO:0005488,GO:0005515,GO:0042802
-
0.00003284
48.0
WLSH2_k127_8974626_2
Phosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP to their respective diphosphate derivatives. Probably excludes non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions
Attaches a formyl group to the free amino group of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus
K00604
-
2.1.2.9
0.00000000000000000000000000000000000003887
146.0
WLSH2_k127_8988148_1
Attaches a formyl group to the free amino group of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus
K00604
-
2.1.2.9
0.000000000002419
68.0
WLSH2_k127_8988148_2
AI-2E family transporter
-
-
-
0.0002646
46.0
WLSH2_k127_9022293_0
ABC-type antimicrobial peptide transport system, permease component
K02004
-
-
0.000000000000000000000000000000007729
134.0
WLSH2_k127_9022293_1
Efflux ABC transporter permease protein
K02004
-
-
0.00000000000000000000000000001033
123.0
WLSH2_k127_903032_0
Bacterial sugar transferase
-
-
-
0.000000000000000000000000000000000000007992
147.0
WLSH2_k127_903032_1
Tetratricopeptide repeat
-
-
-
0.000003135
57.0
WLSH2_k127_9047202_0
Alpha-2-Macroglobulin
K06894
-
-
0.00000000000000000001498
105.0
WLSH2_k127_9047202_1
Belongs to the pirin family
K06911
-
-
0.00009787
45.0
WLSH2_k127_9048469_0
Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit
K02887
-
-
0.0000000000000000000000000000785
120.0
WLSH2_k127_9048469_1
Could be involved in insertion of integral membrane proteins into the membrane
K08998
-
-
0.0000000000000000001623
91.0
WLSH2_k127_9048469_2
IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins
K02520
-
-
0.00000000000000002689
87.0
WLSH2_k127_9048469_3
Belongs to the bacterial ribosomal protein bL35 family
Required for disulfide bond formation in some periplasmic proteins. Acts by transferring its disulfide bond to other proteins and is reduced in the process
-
-
-
0.00000009913
58.0
WLSH2_k127_9136715_0
ATPase, P-type (transporting), HAD superfamily, subfamily IC
PFAM alkyl hydroperoxide reductase Thiol specific antioxidant Mal allergen
K03564
-
1.11.1.15
0.00003723
46.0
WLSH2_k127_9136715_4
copper-exporting ATPase
K01533,K17686
-
3.6.3.4,3.6.3.54
0.0003061
43.0
WLSH2_k127_9183294_0
Protein of unknown function (DUF1800)
-
-
-
0.00000000000000002453
95.0
WLSH2_k127_9229448_0
ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins
Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner
damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage
Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex
K02115
-
-
0.00000000000000000000000000000000000005945
152.0
WLSH2_k127_9408068_3
Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex
K02115
-
-
0.000000213
53.0
WLSH2_k127_9436622_0
Prokaryotic N-terminal methylation motif
K02456,K02650,K02655
-
-
0.0003097
51.0
WLSH2_k127_9442268_0
Non-canonical ABC transporter that contains transmembrane domains (TMD), which form a pore in the membrane, and an ATP-binding domain (NBD), which is responsible for energy generation. Confers resistance against macrolides
The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by UvrB, the UvrA molecules dissociate
The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by UvrB, the UvrA molecules dissociate
K03701
-
-
0.000000000000000000001316
95.0
WLSH2_k127_962310_2
domain protein
-
-
-
0.00000000002823
68.0
WLSH2_k127_9629089_0
Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec)
K01875
-
6.1.1.11
0.00000000000000000000001016
106.0
WLSH2_k127_9640765_0
Alpha-2-Macroglobulin
K06894
-
-
0.00000000000000004936
93.0
WLSH2_k127_9640765_1
Alpha-2-macroglobulin family
K06894
-
-
0.00001857
51.0
WLSH2_k127_9673671_0
This protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site
Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family
-
-
-
0.00000000004039
65.0
WLSH2_k127_9756306_0
Catalyzes the phosphorylation of pyrimidine nucleoside monophosphates at the expense of ATP. Plays an important role in de novo pyrimidine nucleotide biosynthesis. Has preference for UMP and CMP as phosphate acceptors
K13800
-
2.7.4.14
0.0000000179
59.0
WLSH2_k127_9782955_0
sister chromatid segregation
-
-
-
0.0002238
52.0
WLSH2_k127_9875333_0
Belongs to the universal ribosomal protein uS2 family
Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome
K02357
-
-
0.00000000000000000000000000002865
121.0
WLSH2_k127_9875333_2
Domain of unknown function (DUF4870)
-
-
-
0.0001226
49.0
WLSH2_k127_9878616_0
radical SAM domain protein
-
-
-
0.00000000000000002864
83.0
WLSH2_k127_9878616_1
radical SAM domain protein
-
-
-
0.00000000000003847
73.0
WLSH2_k127_9878616_2
radical SAM domain protein
-
-
-
0.000000003954
59.0
WLSH2_k127_9883126_0
it plays a direct role in the translocation of protons across the membrane
K02108
-
-
0.0000000000000000007441
96.0
WLSH2_k127_9900067_0
Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone
Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone
